中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2011年
3期
157-161
,共5页
林碧琛%杨杰%赵玉鸣%葛立宏
林碧琛%楊傑%趙玉鳴%葛立宏
림벽침%양걸%조옥명%갈립굉
牙髓%牙根吸收%恒牙胚缺失乳牙
牙髓%牙根吸收%恆牙胚缺失乳牙
아수%아근흡수%항아배결실유아
Dental pulp%Root Resorption%Primary tooth without permanent tooth germ
目的 研究恒牙胚缺失情况下,乳牙牙髓在其牙根吸收中的作用.方法 通过手术方式建立恒牙胚缺失犬动物模型,定期拍摄X线片观察乳牙根吸收情况.获取乳牙牙根吸收各期颌骨及牙髓样本,采用组织学方法和半定量反转录聚合酶链反应(RT-PCR)分析破牙细胞及其活化因子的分布情况.通过早期乳牙牙髓摘除术验证乳牙牙髓在牙根吸收中的作用.结果 组织学研究结果显示,恒牙胚缺失情况下乳磨牙牙根吸收时,破牙细胞主要在牙根的髓腔侧聚集.半定量RT-PCR结果显示,恒牙牙髓、乳牙牙髓、恒牙胚缺失乳磨牙牙根吸收中、晚期牙髓中NF-κB受体激活蛋白配体mRNA与β-肌动蛋白的灰度值比值分别为0.1314、0.1901、0.2111、0.6058(P＞0.05).早期乳牙牙髓摘除术后,恒牙胚缺失乳牙牙根吸收平均开始于第26周,晚于对照牙(第21周).结论 在恒牙胚缺失乳牙牙根吸收过程中,乳牙牙髓发挥了重要作用.
目的 研究恆牙胚缺失情況下,乳牙牙髓在其牙根吸收中的作用.方法 通過手術方式建立恆牙胚缺失犬動物模型,定期拍攝X線片觀察乳牙根吸收情況.穫取乳牙牙根吸收各期頜骨及牙髓樣本,採用組織學方法和半定量反轉錄聚閤酶鏈反應(RT-PCR)分析破牙細胞及其活化因子的分佈情況.通過早期乳牙牙髓摘除術驗證乳牙牙髓在牙根吸收中的作用.結果 組織學研究結果顯示,恆牙胚缺失情況下乳磨牙牙根吸收時,破牙細胞主要在牙根的髓腔側聚集.半定量RT-PCR結果顯示,恆牙牙髓、乳牙牙髓、恆牙胚缺失乳磨牙牙根吸收中、晚期牙髓中NF-κB受體激活蛋白配體mRNA與β-肌動蛋白的灰度值比值分彆為0.1314、0.1901、0.2111、0.6058(P＞0.05).早期乳牙牙髓摘除術後,恆牙胚缺失乳牙牙根吸收平均開始于第26週,晚于對照牙(第21週).結論 在恆牙胚缺失乳牙牙根吸收過程中,乳牙牙髓髮揮瞭重要作用.
목적 연구항아배결실정황하,유아아수재기아근흡수중적작용.방법 통과수술방식건립항아배결실견동물모형,정기박섭X선편관찰유아근흡수정황.획취유아아근흡수각기합골급아수양본,채용조직학방법화반정량반전록취합매련반응(RT-PCR)분석파아세포급기활화인자적분포정황.통과조기유아아수적제술험증유아아수재아근흡수중적작용.결과 조직학연구결과현시,항아배결실정황하유마아아근흡수시,파아세포주요재아근적수강측취집.반정량RT-PCR결과현시,항아아수、유아아수、항아배결실유마아아근흡수중、만기아수중NF-κB수체격활단백배체mRNA여β-기동단백적회도치비치분별위0.1314、0.1901、0.2111、0.6058(P＞0.05).조기유아아수적제술후,항아배결실유아아근흡수평균개시우제26주,만우대조아(제21주).결론 재항아배결실유아아근흡수과정중,유아아수발휘료중요작용.
Objective To investigate the role of pulp in the root resorption of primary teeth without permanent tooth germs. Methods The animal model without permanent tooth germs was established by surgery in Beagle dog. The root resorption was observed by taking periapical radiographs periodically. The samples of mandibular bone and pulp at different resorption stages were collected. The distribution of odontoclasts and the activating factor was analyzed by histological staining and semiquantitative reverse transcriptase polymerase chain reaction(RT-PCR). The role of pulp in the root resorption of primary teeth was tested by early pulpectomy. Results In the root resorption of primary molars without permanent teeth germs, a large number of odontoclasts were present on the pulpal surface of the root canal. Semiquantification RT-PCR showed that the ratios of the expression of receptor activator of NF-κB ligand (RANKL) mRNA and β-actin in the pulp of permanent teeth and primary teeth without permanent teeth germ during different periods of root resorption are 0. 1314, 0. 1901, 0. 2111 and 0. 6058 ( P ＞ 0. 05 ). The root resorption of primary teeth without permanent teeth germs in test groups was about 5 weeks later than that of control group. Conclusions The pulp of primary tooth played an important role in the root resorption of primary tooth without permanent tooth germ.
