中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
8期
673-674
,共2页
周海红%刘军%肖颂华%刘运林%邢诒刚%赵斌
週海紅%劉軍%肖頌華%劉運林%邢詒剛%趙斌
주해홍%류군%초송화%류운림%형이강%조빈
碱性成纤维细胞生长因子%大鼠%放射%学习记忆
堿性成纖維細胞生長因子%大鼠%放射%學習記憶
감성성섬유세포생장인자%대서%방사%학습기억
Basic fibroblast growth factor%Rat%Radiation%Learning and memory ability
目的 探讨外源性碱性成纤维细胞生长因子(bFGF)对放射后大鼠学习记忆能力的影响.方法 利用分割照射建立的迟发性放射性脑损伤(RIB)模型鼠24只,采用尾静脉直接注射给药法给予外源性bFGF,3个月后进行学习记忆能力检测,同时观察脑组织病理变化及FGF受体1(FGFR1)表达情况.结果 bFGF组平均寻台潜伏期[(56.57±4.29)s]和第一象限路径与总路径长度百分比[(21.57±1.47)%]均较模型组[(118.24±10.79)s,(6.95±0.75)%]有显著改善(P<0.01);HE染色海马区域完整细胞计数bFGF组[(115.61±12.82)个]较模型组[(70.86±8.51)个]明显增多(P<0.01);IHC结果显示,bFGF治疗组海马内FGFR1表达水平(31.50±1.59)较模型组(12.03±1.68)明显增高(P<0.01).结论 外源性bFGF可通过诱导FGFR1表达对RIB模型鼠学习记忆能力起到一定的保护作用.
目的 探討外源性堿性成纖維細胞生長因子(bFGF)對放射後大鼠學習記憶能力的影響.方法 利用分割照射建立的遲髮性放射性腦損傷(RIB)模型鼠24隻,採用尾靜脈直接註射給藥法給予外源性bFGF,3箇月後進行學習記憶能力檢測,同時觀察腦組織病理變化及FGF受體1(FGFR1)錶達情況.結果 bFGF組平均尋檯潛伏期[(56.57±4.29)s]和第一象限路徑與總路徑長度百分比[(21.57±1.47)%]均較模型組[(118.24±10.79)s,(6.95±0.75)%]有顯著改善(P<0.01);HE染色海馬區域完整細胞計數bFGF組[(115.61±12.82)箇]較模型組[(70.86±8.51)箇]明顯增多(P<0.01);IHC結果顯示,bFGF治療組海馬內FGFR1錶達水平(31.50±1.59)較模型組(12.03±1.68)明顯增高(P<0.01).結論 外源性bFGF可通過誘導FGFR1錶達對RIB模型鼠學習記憶能力起到一定的保護作用.
목적 탐토외원성감성성섬유세포생장인자(bFGF)대방사후대서학습기억능력적영향.방법 이용분할조사건립적지발성방사성뇌손상(RIB)모형서24지,채용미정맥직접주사급약법급여외원성bFGF,3개월후진행학습기억능력검측,동시관찰뇌조직병리변화급FGF수체1(FGFR1)표체정황.결과 bFGF조평균심태잠복기[(56.57±4.29)s]화제일상한로경여총로경장도백분비[(21.57±1.47)%]균교모형조[(118.24±10.79)s,(6.95±0.75)%]유현저개선(P<0.01);HE염색해마구역완정세포계수bFGF조[(115.61±12.82)개]교모형조[(70.86±8.51)개]명현증다(P<0.01);IHC결과현시,bFGF치료조해마내FGFR1표체수평(31.50±1.59)교모형조(12.03±1.68)명현증고(P<0.01).결론 외원성bFGF가통과유도FGFR1표체대RIB모형서학습기억능력기도일정적보호작용.
Objective To explore the therapeutic effects of basic fibroblast growth factor(bFGF) on learning and memory ability of post-radiation rats. Methods 24 Sprague-Dawley rats were treated with 30 Gy fractionated irradiation and then randomly divided into 2 groups. Exogenous hFGF and normal sodium were admin-istered intravenously through caudal vein after modeling. Morris maze tests were taken to study the learning and memory ability of rats 3 months after injection. The pathologic feature was studied by using H&E staining and IHC staining of FGF receptor 1 (FGFRI). Results Statistic differences of average escape latency and search strategy were found between model group [(118.24±10.79) s, (6.95±0.75)%] and bFGF group[(56.57±4.29) s, (21.57±1.47) %, P<0.01]. Intact cell number in hippocampus was more in bFGF group (115.61±12.82) than model group (70.86±8.51, P<0.01). There was weak expression of FGFR1 in hippocampus of model group(12.03±1.68) , which were fewer than bFGF group (31.50±1.59, P<0.01). Conclusion Exogenous bFGF injected intravenously through caudal vein has therapeutic effcets on learning and memory ability of post-radiation rats by inducing the expression of FGFR1.
