CAJ | 학술논문

目的观察会雀异黄素对体外培养的正常人皮肤成纤维细胞生长和胶原合成的影响.方法采用噻哗蓝(MTT)比色法检测不同浓度金雀异黄索对体外培养的人皮肤成纤维细胞存活力的影响,并绘制细胞的生长曲线;流式细胞仪检测细胞周期的变化;RT-PCR检测不同浓度金雀异黄素对成纤维细胞⒈型胶原mRNA表达的影响.结果 0.03125,0.0625,0.125,0.25,0.5,1 mg/L金雀异黄素作用24 h时,成纤维细胞增殖率分别为97.7%,113.8%,132.5%,116.4%,94.5%和83.3%.当金雀异黄素质量浓度大于0.5 mg/L,对成纤维细胞的增殖表现出抑制作用.浓度在0.0625～0.25 mg/L之间时,对成纤维细胞的增殖表现出促进作用.0.0625、0.125、0.25 mg/L金雀异黄素作用于成纤维细胞后,S期(41.15%±2.88%,61.89%±3.16%,48.18%±1.68%)和G2期(9.76%±3.99%,10.40%±0.54%,7.46%±2.47%)细胞明显高于对照组(S期为30.12%±0.60%,G2期为0.61%±0.16%),两组比较,差异均有统计学意义(P均＜0.05);而G1期细胞(49.08%±3.58%,30.04%±1.89%,44.36%±3.92%)明显低于对照组(69.27%±0.73%),两组比较,差异均有统计学意义(P均＜0.05);3个浓度组Ⅰ型胶原mRNA的表达(0.4814±0.0138,0.5767±0.0291,0.5675±0.0272)均高于对照组(0.4101±0.0236),两组比较,差异均有统计学意义(P均＜0.01).1 mg/L和0.5 mg/L的金雀异黄素作用成纤维细胞后,Ⅰ型胶原mRNA的表达(0.1662±0.0165,0.2017±0.0203)低于对照组,两组比较,差异均有统计学意义(P均＜0.01).结论一定浓度的金雀异黄素可以促进正常人皮肤成纤维细胞的增殖和生长,并促进成纤维细胞Ⅰ型胶原mRNA的表达.
목적 관찰회작이황소대체외배양적정상인피부성섬유세포생장화효원합성적영향.방법 채용새화람(MTT)비색법검측불동농도금작이황색대체외배양적인피부성섬유세포존활력적영향,병회제세포적생장곡선;류식세포의검측세포주기적변화;RT-PCR검측불동농도금작이황소대성섬유세포⒈형효원mRNA표체적영향.결과 0.03125,0.0625,0.125,0.25,0.5,1 mg/L금작이황소작용24 h시,성섬유세포증식솔분별위97.7%,113.8%,132.5%,116.4%,94.5%화83.3%.당금작이황소질량농도대우0.5 mg/L,대성섬유세포적증식표현출억제작용.농도재0.0625～0.25 mg/L지간시,대성섬유세포적증식표현출촉진작용.0.0625、0.125、0.25 mg/L금작이황소작용우성섬유세포후,S기(41.15%±2.88%,61.89%±3.16%,48.18%±1.68%)화G2기(9.76%±3.99%,10.40%±0.54%,7.46%±2.47%)세포명현고우대조조(S기위30.12%±0.60%,G2기위0.61%±0.16%),량조비교,차이균유통계학의의(P균＜0.05);이G1기세포(49.08%±3.58%,30.04%±1.89%,44.36%±3.92%)명현저우대조조(69.27%±0.73%),량조비교,차이균유통계학의의(P균＜0.05);3개농도조Ⅰ형효원mRNA적표체(0.4814±0.0138,0.5767±0.0291,0.5675±0.0272)균고우대조조(0.4101±0.0236),량조비교,차이균유통계학의의(P균＜0.01).1 mg/L화0.5 mg/L적금작이황소작용성섬유세포후,Ⅰ형효원mRNA적표체(0.1662±0.0165,0.2017±0.0203)저우대조조,량조비교,차이균유통계학의의(P균＜0.01).결론 일정농도적금작이황소가이촉진정상인피부성섬유세포적증식화생장,병촉진성섬유세포Ⅰ형효원mRNA적표체.
Objective To study the effects of genistein on the biological characteristics of and collagen synthesis by human skin fibroblasts in vitro.Methods Human skin fibroblasts(HSFBs)were obtained from the prepuce of healthy adolescents,and suhjected to primary culture.Atier 5 to 15 passages of culture,HSFBs were treated with various concentmtions(0.03125,0.0625,0.125,0.25,0.5,1mg/L)of genistein for different durations.The potential of cell proliferation was detected by MTT assay and growth curves were drawn for HSFBs.Flow cytometry(FCM)and RT-PCR were used to estimate cell cycle phases and mRNA expression of type Ⅰ procollagen.respectively.Results The proliferation rate of HSFBs was 97.7%,113.8%,132.5%,116.4%,94.5%and 83.3%after treatment with genistein of 0.03125,0.0625,0.125,0.25,0.5 and 1 mg/L,respectively.The genistein of more than 0.5 mg/L displayed an inhibitive effect on the proliferation of HSFBs.while that between 0.0625 and 0.25 mg/L showed a promotive effect.Atier treatment with genistein at 0.0625,0.1 25 and 0.25 mg/L,the percentage of HSFBs in S phase and G2 phase significantly increased compared with untreated HSFBs(S phase:41.15%±2.88%,61.89%±3.16%,48.18%±1.68%vs30.12%±0.60%,P＜0.05;G2 phase:9.76%±3.99%,10.40%±0.54%,7.46%±2.47%vs 0.61%±0.16%,P＜0.05).Compared with the untreated HSFBs.the relative mRNA expression level of type Ⅰ procollagen was increased with genistein of 0.0625,0.125 and 0.25 mg/L(0.4814±0.0138,0.5767±0.0291,0.5675±0.0272 vs 0.4101±0.0236,P＜0.01),but decreased with genistein of Ⅰ and 0.5 mg/L(0.1662±0.0165 and 0.2017±0.0203 vs 0.4101±0.0236,P＜0.01).ConclusionCertain concentrations of genistein could enhance the proliferation and growth of as well as mRNA expression of type Ⅰ procollagen in HSFBs.