中华肝脏病杂志
中華肝髒病雜誌
중화간장병잡지
CHINESE JOURNAL OF HEPATOLOGY
2009年
7期
520-525
,共6页
舒宏%康晓楠%李梅%郭坤%孙璐%李山%谢丽%邓敬桓%秦雪%刘银坤
舒宏%康曉楠%李梅%郭坤%孫璐%李山%謝麗%鄧敬桓%秦雪%劉銀坤
서굉%강효남%리매%곽곤%손로%리산%사려%산경환%진설%류은곤
癌,肝细胞%蛋白质组%双向电泳%结合珠蛋白
癌,肝細胞%蛋白質組%雙嚮電泳%結閤珠蛋白
암,간세포%단백질조%쌍향전영%결합주단백
Carcinoma,hepatocellular,Proteomics%Two-dimensional electrophoresis%Haptoglobin
目的 比较肝癌发生不同病理阶段血清蛋白质组的表达变化情况,从中寻找特异性标志物.方法 用双向凝胶电泳分离正常人,慢性肝炎、肝硬化和肝癌患者的血清蛋白质,结合质谱技术对差异点进行鉴定; Western blot检测结合珠蛋白β链以验证蛋白质水平的表达.计量资料数据以均数±标准差(x±s)表示,采用方差分析和LSD检验进行两两比较.结果 4组血清的双向电泳图谱经软件匹配分析,并与基质辅助激光解析电离飞行时间质谱相结合,成功鉴定出结合珠蛋白,SAA1、SP40等30个差异蛋白质点,K cluster聚类分析不同的变化模式.在差异蛋白质点中,7个蛋白质点均为结合珠蛋白,呈现由正常→肝炎→肝硬化持续下调、到肝癌又上调的模式.Western blot证实结合珠蛋白β链的表达与双向电泳表达相一致.结论 在肝癌发生的动态过程中各疾病阶段的血清蛋白质差异表达有4种明显变化模式,可能为肝癌的早期诊断和预后提供依据,与肝癌发生发展相关的结合珠蛋白很可能是肝硬化发展到肝癌的一个潜在早期诊断标志物.
目的 比較肝癌髮生不同病理階段血清蛋白質組的錶達變化情況,從中尋找特異性標誌物.方法 用雙嚮凝膠電泳分離正常人,慢性肝炎、肝硬化和肝癌患者的血清蛋白質,結閤質譜技術對差異點進行鑒定; Western blot檢測結閤珠蛋白β鏈以驗證蛋白質水平的錶達.計量資料數據以均數±標準差(x±s)錶示,採用方差分析和LSD檢驗進行兩兩比較.結果 4組血清的雙嚮電泳圖譜經軟件匹配分析,併與基質輔助激光解析電離飛行時間質譜相結閤,成功鑒定齣結閤珠蛋白,SAA1、SP40等30箇差異蛋白質點,K cluster聚類分析不同的變化模式.在差異蛋白質點中,7箇蛋白質點均為結閤珠蛋白,呈現由正常→肝炎→肝硬化持續下調、到肝癌又上調的模式.Western blot證實結閤珠蛋白β鏈的錶達與雙嚮電泳錶達相一緻.結論 在肝癌髮生的動態過程中各疾病階段的血清蛋白質差異錶達有4種明顯變化模式,可能為肝癌的早期診斷和預後提供依據,與肝癌髮生髮展相關的結閤珠蛋白很可能是肝硬化髮展到肝癌的一箇潛在早期診斷標誌物.
목적 비교간암발생불동병리계단혈청단백질조적표체변화정황,종중심조특이성표지물.방법 용쌍향응효전영분리정상인,만성간염、간경화화간암환자적혈청단백질,결합질보기술대차이점진행감정; Western blot검측결합주단백β련이험증단백질수평적표체.계량자료수거이균수±표준차(x±s)표시,채용방차분석화LSD검험진행량량비교.결과 4조혈청적쌍향전영도보경연건필배분석,병여기질보조격광해석전리비행시간질보상결합,성공감정출결합주단백,SAA1、SP40등30개차이단백질점,K cluster취류분석불동적변화모식.재차이단백질점중,7개단백질점균위결합주단백,정현유정상→간염→간경화지속하조、도간암우상조적모식.Western blot증실결합주단백β련적표체여쌍향전영표체상일치.결론 재간암발생적동태과정중각질병계단적혈청단백질차이표체유4충명현변화모식,가능위간암적조기진단화예후제공의거,여간암발생발전상관적결합주단백흔가능시간경화발전도간암적일개잠재조기진단표지물.
Objectives To compare the 2-DE profiles for serum proteins of different pathological stages during hepatocareinogenesis. Methods Sera from hepatoeellular carcinoma patients, cirrhosis patients,chronic hepatitis patients and healthy controls were collected. After sonication, albumin and immunogiobulin (IgG) depletion, and desalination, sera were subjected to 2-DE, the differential protein spots were identified by MALDI-TOF-MS. Western blot was used to validate these differentially expressed proteins. Results 2-DE sera protein profiles were obtained fi'om the patient suffering from HCC, liver cirrhosis, chronic hepatitis,healthy controls in each group. From optimized 2-DE gel images of the above groups, 96 protein spots with more than 2-fold difference in intensity between the two groups were selected by image master 6.0 software,differential proteins including haptoglobin, SAAI and SP40 were identified by MALDI-TOF-MS/MS. 7 different spots within more than 30 protein spots belonged to the same haptogiobin family. The differential expression of haptoglobin was confirmed by western blot. Conclusions Four protein expression patterns have been identified during the pathological stages of hepatocarcinogenesis. Haptoglobin is significantly increased from liver cirrhosis to HCC. It implies that haptoglobin might be a potential biomarker in the early diagnosis of liver cancer.
