中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2010年
5期
507-509
,共3页
细胞凋亡%人类解旋酶样转录因子(HLTF)%细胞色素C
細胞凋亡%人類解鏇酶樣轉錄因子(HLTF)%細胞色素C
세포조망%인류해선매양전록인자(HLTF)%세포색소C
Cell apoptosis%Helicase-like transcription factor (HLTF)%Cytochrome C
目的 探讨人类解旋酶样转录因子(HLTF)对辐射诱导细胞凋亡的影响及其机制.方法 将空质粒和带FLAG标签的HLTF表达质粒分别转染人肺癌细胞A549,60Coγ射线照射观察细胞凋亡变化.用Western blot检测HLTF在细胞核和胞质内水平的变化及线粒体细胞色素C的释放.结果 HLTF转染可促进γ射线诱导的细胞凋亡,凋亡率为(32.2±2.1)%,高于空载体转染组(11.4±2.3)%和Mock转染组(11.1±1.8)%(F=101.85,P＜0.01).辐射后HLTF在胞质内的表达水平显著提高.HLTF转染组胞质内细胞色素C的水平亦显著提高.结论 HLTF可促进辐射损伤诱导的细胞凋亡,其部分机制可能是通过提高核质转位以及影响线粒体细胞色素C的释放来实现的.
目的 探討人類解鏇酶樣轉錄因子(HLTF)對輻射誘導細胞凋亡的影響及其機製.方法 將空質粒和帶FLAG標籤的HLTF錶達質粒分彆轉染人肺癌細胞A549,60Coγ射線照射觀察細胞凋亡變化.用Western blot檢測HLTF在細胞覈和胞質內水平的變化及線粒體細胞色素C的釋放.結果 HLTF轉染可促進γ射線誘導的細胞凋亡,凋亡率為(32.2±2.1)%,高于空載體轉染組(11.4±2.3)%和Mock轉染組(11.1±1.8)%(F=101.85,P＜0.01).輻射後HLTF在胞質內的錶達水平顯著提高.HLTF轉染組胞質內細胞色素C的水平亦顯著提高.結論 HLTF可促進輻射損傷誘導的細胞凋亡,其部分機製可能是通過提高覈質轉位以及影響線粒體細胞色素C的釋放來實現的.
목적 탐토인류해선매양전록인자(HLTF)대복사유도세포조망적영향급기궤제.방법 장공질립화대FLAG표첨적HLTF표체질립분별전염인폐암세포A549,60Coγ사선조사관찰세포조망변화.용Western blot검측HLTF재세포핵화포질내수평적변화급선립체세포색소C적석방.결과 HLTF전염가촉진γ사선유도적세포조망,조망솔위(32.2±2.1)%,고우공재체전염조(11.4±2.3)%화Mock전염조(11.1±1.8)%(F=101.85,P＜0.01).복사후HLTF재포질내적표체수평현저제고.HLTF전염조포질내세포색소C적수평역현저제고.결론 HLTF가촉진복사손상유도적세포조망,기부분궤제가능시통과제고핵질전위이급영향선립체세포색소C적석방래실현적.
Objective To study the effects of overexpression of helicase-like transcription factor (HLTF) on radiation-induced apoptosis of human lung carcinoma cells.Methods Human lung carcinoma cellline A549 were cultured,transfected with FLAG-tagged HLTF expression plasmid pcDNA3.1/HLTF-FLAG (HLTF gene transfection group) ,empty plasmid vector pcDNA3.1 (empty vector group) ,and mock transfection group,respectively,and then exposed to 60Co γ-ray irradiation.Cell apoptosis was observed by flow cytometry 48 h post-irradiation.Western blotting was used to detect the levels of HLTF in the nucleus and cytoplasm and the release of cytochrome C from mitochondria into cytoplasm.Results The apoptosis rate of the HLTF gene group was (32.2 ± 2.1) %,significantly higher than those of the empty vector group [(11.4±2.3)%] and mock transfection group [(11.1 ±1.8)%] (F=101.85,P＜0.05).The level of cytochrome C in the cytoplasm of the HLTF gene group was significantly higher than that of the empty plasmid group.Conclusions HLTF protein sensitizes human lung carcinoma cells to radiation-induced apoptosis,partly by promoting the nuclear-cytoplasma transition of HLTF protein and the increased release of cytochrome C.
