中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2008年
6期
546-548
,共3页
张煜%崔尧元%吴伟忠%孙瑞霞
張煜%崔堯元%吳偉忠%孫瑞霞
장욱%최요원%오위충%손서하
胶质瘤%细胞增殖%细胞周期%STAT3
膠質瘤%細胞增殖%細胞週期%STAT3
효질류%세포증식%세포주기%STAT3
Glioma%Cell proliferation%Cell cycle%STAT3
目的 探讨AG490阻断STAT3信号通路对人胶质瘤细胞增殖和细胞周期的影响.方法 用不同浓度的AG490作用于体外培养的人胶质瘤细胞株U87、U251;用免疫荧光细胞化学染色观察肿瘤细胞STAT3和激活态p-STAT3的表达;Western blot验证AG490对STAT3信号通路的阻断情况;Sulforhodamine B染色观察肿瘤细胞增殖的改变;流式细胞技术分析细胞周期的变化. 结果 STAT3蛋白在胶质瘤细胞胞浆中表达,而P-STAT3则在细胞核中表达.AG490作用胶质瘤细胞后可使p-STAT3表达下降,而STAT3表达不受影响.AG490阻断STAT3信号通路后,胶质瘤细胞的增殖受到显著抑制,该抑制作用与剂量及时间存在一定关系.AG490作用后胶质瘤的细胞周期出现阻滞.结论 应用AG490阻断STAT3通路能够导致胶质瘤细胞增殖的抑制和细胞周期的阻滞.针对STAT3信号通路的研究可能为胶质瘤的治疗提供更加有效的方法.
目的 探討AG490阻斷STAT3信號通路對人膠質瘤細胞增殖和細胞週期的影響.方法 用不同濃度的AG490作用于體外培養的人膠質瘤細胞株U87、U251;用免疫熒光細胞化學染色觀察腫瘤細胞STAT3和激活態p-STAT3的錶達;Western blot驗證AG490對STAT3信號通路的阻斷情況;Sulforhodamine B染色觀察腫瘤細胞增殖的改變;流式細胞技術分析細胞週期的變化. 結果 STAT3蛋白在膠質瘤細胞胞漿中錶達,而P-STAT3則在細胞覈中錶達.AG490作用膠質瘤細胞後可使p-STAT3錶達下降,而STAT3錶達不受影響.AG490阻斷STAT3信號通路後,膠質瘤細胞的增殖受到顯著抑製,該抑製作用與劑量及時間存在一定關繫.AG490作用後膠質瘤的細胞週期齣現阻滯.結論 應用AG490阻斷STAT3通路能夠導緻膠質瘤細胞增殖的抑製和細胞週期的阻滯.針對STAT3信號通路的研究可能為膠質瘤的治療提供更加有效的方法.
목적 탐토AG490조단STAT3신호통로대인효질류세포증식화세포주기적영향.방법 용불동농도적AG490작용우체외배양적인효질류세포주U87、U251;용면역형광세포화학염색관찰종류세포STAT3화격활태p-STAT3적표체;Western blot험증AG490대STAT3신호통로적조단정황;Sulforhodamine B염색관찰종류세포증식적개변;류식세포기술분석세포주기적변화. 결과 STAT3단백재효질류세포포장중표체,이P-STAT3칙재세포핵중표체.AG490작용효질류세포후가사p-STAT3표체하강,이STAT3표체불수영향.AG490조단STAT3신호통로후,효질류세포적증식수도현저억제,해억제작용여제량급시간존재일정관계.AG490작용후효질류적세포주기출현조체.결론 응용AG490조단STAT3통로능구도치효질류세포증식적억제화세포주기적조체.침대STAT3신호통로적연구가능위효질류적치료제공경가유효적방법.
Objective To study the effect of AG490 on cell proliferation and cycle of human glioma cell lines by inhibiting the STAT3 signal pathway. Methods Glioma cell lines U87 and U251 were treated with AG490, a Janus kinase (JAK) inhibitor, at different concentrations and for various durations. STAT3 and phospho-STAT3 (p-STAT3) proteins were detected by immanocytochemical staining. The blocking effect of AG490 on STAT3 signal pathway was verified by means of Western blot which displayed the expressions of STAT3 and p-STAT3 proteins in glioma cells. The cell proliferation of glioma cell lines was checked by sulforhodamine B (SRB) assay. FCM was applied to analyze the change of cell cycle. Results The expression of STAT3 was located in cytoplasm of glioma cells while p-STAT3 in the cell nucleus. The expression of p-STAT3 could be inhibited by AG490 in U251 and U87 cell lines while STAT3 stayed unchanged. And AG490 appeared to significantly inhibit the proliferation of glioma cell lines in a time-and concen ration-dependent manner. Moreover, it leads to cell cycle arrest of glioma cells in vitro. Conclusion That AG490 suppresses the cell proliferation and arrests the cell cycle of glioma cell lines in vitro indicates that the STAT3 signaling pathway plays an important role in the development of glioma. STAT3 is possible to be a promising target in the control of glloma.
