CAJ | 학술논문

三氯醋酸产生于工业废弃物及水的氯化过程,是重要的环境污染物.对于它的检测,常用的方法比较繁琐.应用血红蛋白-蒙脱土膜修饰热解石墨电极,将血红蛋白包埋于修饰在热解石墨电极上的蒙脱土内,进行循环伏安实验,可以得到血红蛋白的直接电化学响应,在pH 4.0 0.1 mol/L柠檬酸缓冲液中,于-0.292 Ⅴ处出现一对准可逆的氧化还原峰(相对于饱和甘汞电极,vs.SCE).经紫外-可见光谱和傅利叶红外光谱测定,包埋在蒙脱土膜内的血红蛋白仍保持与其天然状态相似的二级结构,而且将三氯醋酸加入上述体系中,发现该修饰电极对三氯醋酸具有催化功能.表观米氏常数的测定结果显示,该参数可作为这种电极与底物三氯醋酸作用的酶性质的表征.同时血红蛋白-蒙脱土膜修饰热解石墨电极性质稳定,对于三氯醋酸,显示出了酶样的催化作用特性,特异性高.因此水中虽然存在一些浓度高于三氯醋酸三倍的相关干扰物质的同时,该电极仍显示出了良好的选择性,并具有方便、快捷的特点.还从电化学的角度出发,探讨了血红蛋白-蒙脱土膜的修饰电极在环境污染物三氯醋酸检测中应用的可能性.
삼록작산산생우공업폐기물급수적록화과정,시중요적배경오염물.대우타적검측,상용적방법비교번쇄.응용혈홍단백-몽탈토막수식열해석묵전겁,장혈홍단백포매우수식재열해석묵전겁상적몽탈토내,진행순배복안실험,가이득도혈홍단백적직접전화학향응,재pH 4.0 0.1 mol/L저몽산완충액중,우-0.292 Ⅴ처출현일대준가역적양화환원봉(상대우포화감홍전겁,vs.SCE).경자외-가견광보화부리협홍외광보측정,포매재몽탈토막내적혈홍단백잉보지여기천연상태상사적이급결구,이차장삼록작산가입상술체계중,발현해수식전겁대삼록작산구유최화공능.표관미씨상수적측정결과현시,해삼수가작위저충전겁여저물삼록작산작용적매성질적표정.동시혈홍단백-몽탈토막수식열해석묵전겁성질은정,대우삼록작산,현시출료매양적최화작용특성,특이성고.인차수중수연존재일사농도고우삼록작산삼배적상관간우물질적동시,해전겁잉현시출료량호적선택성,병구유방편、쾌첩적특점.환종전화학적각도출발,탐토료혈홍단백-몽탈토막적수식전겁재배경오염물삼록작산검측중응용적가능성.
Stable thin films made from sodium montmorillonite with incorporated hemoglobin (Hb)on pyrolytic graphite (PG) electrodes were characterized by electrochemical analysis. Cyclic voltammetry (CV) of Hb-montmorillonite films showed a pair of well defined and nearly reversible peaks at about - 0. 292 V vs. saturated calomel electrode (SCE) at pH 4.0, showing greatly facilitated direct electron transfer between Hb and PG electrodes in montmorillonite films. UV-Vis absorption spectroscopy and FT-IR spectroscopy suggested that Hb keeps its secondary structure similar to its native state when incorporated in the film. Moreover, this modified electrode exhibited the catalysis activity to trichloroacetic acid (TCA), and the TCA reductive peak current is in proportion to its concentration. The apparent Michaelis-Menten constant was about 2.37 mmol/L.The methods is prospective based on Hb-montmorillonite modified electrodes for detection of TCA.The possible interference in real sample analyses is discussed.