神经科学通报（英文版）
神經科學通報（英文版）
신경과학통보（영문판）
NEUROSCIENCE BULLETIN
2008年
5期
283-287
,共5页
徐清%程慧雯%何慧琼%刘志睿%贺明%杨宏天%周智磊%吉永华
徐清%程慧雯%何慧瓊%劉誌睿%賀明%楊宏天%週智磊%吉永華
서청%정혜문%하혜경%류지예%하명%양굉천%주지뢰%길영화
rNav1.3%电压门控钠通道%糖基化%双电极电压钳%爪蟾卵母细胞
rNav1.3%電壓門控鈉通道%糖基化%雙電極電壓鉗%爪蟾卵母細胞
rNav1.3%전압문공납통도%당기화%쌍전겁전압겸%조섬란모세포
rNavl.3%voltage-gated sodium channel%glycosylation%two-electrode voltage clamp%Xenopus oocyte
目的 观察糖基化对rNav1.3电压门控性质的影响.方法 将rNav1.3在爪蟾卵母细胞中表达,衣霉素抑制其糖基化,双电极电压钳记录全细胞电流,Origin软件分析处理数据.对照组为未去糖基化的rNav1.3.结果 与糖基化的rNav1.3相比,去糖基化的rNav1.3稳态激活曲线向去极化方向偏移约10 mV,稳态失活曲线向超极化方向偏移约8 mV.结论 糖基化修饰改变rNav1.3的电压门控性质,异化其生理功能.
目的 觀察糖基化對rNav1.3電壓門控性質的影響.方法 將rNav1.3在爪蟾卵母細胞中錶達,衣黴素抑製其糖基化,雙電極電壓鉗記錄全細胞電流,Origin軟件分析處理數據.對照組為未去糖基化的rNav1.3.結果 與糖基化的rNav1.3相比,去糖基化的rNav1.3穩態激活麯線嚮去極化方嚮偏移約10 mV,穩態失活麯線嚮超極化方嚮偏移約8 mV.結論 糖基化脩飾改變rNav1.3的電壓門控性質,異化其生理功能.
목적 관찰당기화대rNav1.3전압문공성질적영향.방법 장rNav1.3재조섬란모세포중표체,의매소억제기당기화,쌍전겁전압겸기록전세포전류,Origin연건분석처리수거.대조조위미거당기화적rNav1.3.결과 여당기화적rNav1.3상비,거당기화적rNav1.3은태격활곡선향거겁화방향편이약10 mV,은태실활곡선향초겁화방향편이약8 mV.결론 당기화수식개변rNav1.3적전압문공성질,이화기생리공능.
Objective To examine the effect of deglycosylation on gating properties of rNavl.3.Methods rNavl.3 was expressed in Xenopus oocyte,with glycosylation inhibition by using tunicamycin.Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software.Those of glycosylated rNavl.3 were kept as control.Results Compared with glycosylated ones,the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV,while inactivation curve was negatively shifted by about 8mV.Conclusion Glycosylation altered the gating properties of rNav1.3 and contributed to the functional diversity.
