CAJ | 학술논문

目的探讨大鼠全脑缺血/再灌注损伤后银杏叶提取物(EGb761)减轻脑水肿的机制.方法四血管法制作全脑缺血/再灌注模型的40只雄性SD大鼠,随机分为假手术组、缺血/再灌注组、小剂量EGb761治疗组、中剂量EGb761治疗组和大剂量EGb761治疗组,每组8只;观察脑组织含水量、血清S100B、丙二醛(MDA)和超氧化物歧化酶(SOD)含量.结果与缺血/再灌注组相比,EGb761治疗能降低脑组织含水量、血清S100B蛋白和MDA含量,提升血清SOD含量.结论 EGb761可减轻全脑缺血/再灌注损伤后大鼠的脑水肿,其机制可能与血清S100B蛋白含量下降和抗自由基损伤有关,并且呈剂量依赖性.
목적 탐토대서전뇌결혈/재관주손상후은행협제취물(EGb761)감경뇌수종적궤제.방법 사혈관법제작전뇌결혈/재관주모형적40지웅성SD대서,수궤분위가수술조、결혈/재관주조、소제량EGb761치료조、중제량EGb761치료조화대제량EGb761치료조,매조8지;관찰뇌조직함수량、혈청S100B、병이철(MDA)화초양화물기화매(SOD)함량.결과 여결혈/재관주조상비,EGb761치료능강저뇌조직함수량、혈청S100B단백화MDA함량,제승혈청SOD함량.결론 EGb761가감경전뇌결혈/재관주손상후대서적뇌수종,기궤제가능여혈청S100B단백함량하강화항자유기손상유관,병차정제량의뢰성.
Objective To investigate the mechanism of ginkgo biloba extract (EGb761) on the decrease in brain edema after global cerebral ischemia-reperfusion injury in rats. Methods The global cerebral ischemia-reperfusion model was established by the occlusion of the 4 vessels, reperfusion 6 hours after ischemia 20 minutes. 40 SD rats were randomly divided into 5 groups, each group has 8 rats: sham operated group, ischemia-reperfusion group,low dose EGb761 treatment group,medium dose EGb761 treatment group and high dose EGb761 treatment group. The levels of S100B, Malondialdehyde (MDA), superoxide dismutase (SOD) in serum and brain water content were measured. Results Compared with the ischemia-reperfusion group, EGb761 decreased the brain water content and the level of S100B, MDA in serum, enhanced the level of SOD in serum. Conclusion EGb761 can reduce brain edema after global cerebral ischemia-reperfusion injury in rats through decreasing the level of S100B and counteracting free radical damage, with dose-depended effect.