CAJ | 학술논문

以摇床培养的木霉菌丝为材料,采用液氮研磨等方法提取到高质量的基因组DNA,能够用于AFLP实验分析,探索和优化酶切一连接反应、预扩增和选择性扩增反应过程中的关键因素,建立了适合木霉AFLP的最佳试验体系和反应条件.利用该技术体系对不同时期保存的菌株及融合子进行AFLP指纹分析,发现不同时期保存的同一菌株指纹相同,融合子指纹兼有亲本菌株的特性.通过对木霉AFLP条件的探索和研究,建立了木霉基因组AFLP反应体系,得到的扩增多态性条带完全能够用于木霉遗传稳定性和多样性分析.
이요상배양적목매균사위재료,채용액담연마등방법제취도고질량적기인조DNA,능구용우AFLP실험분석,탐색화우화매절일련접반응、예확증화선택성확증반응과정중적관건인소,건립료괄합목매AFLP적최가시험체계화반응조건.이용해기술체계대불동시기보존적균주급융합자진행AFLP지문분석,발현불동시기보존적동일균주지문상동,융합자지문겸유친본균주적특성.통과대목매AFLP조건적탐색화연구,건립료목매기인조AFLP반응체계,득도적확증다태성조대완전능구용우목매유전은정성화다양성분석.
In order to provide a basis for identification and diversity analysis of Trichoderma spp.through AFLP reaction system,improved method was used to extract high quality genomic DNA with hypha from Trichoderma spp.through liquid nitrogen rubbing,which could be used in amplified fragment length polymorphism(AFLP)analysis,The key factors were explored and optimized among enzyme-digested reaction,ligation,pre-amplification and selective amplification.AFLP fingerprinting of strains stored in different periods and the fusions was analysed with this system.The results showed that the fingerprinting of the same strain in different periods was the same,and the fingerprinting of the fusions had the characters from the parents.Therefore,it was a useful tool to research the identification and genetic diversity in Trichoderma spp.through optimized AFLP system.