CAJ | 학술논문

将脐带血单个核细胞与包埋有兔骨髓间充质干细胞的海藻酸钙微胶珠在3种不同的培养液中进行了7 d的体外静态共培养.每24 h进行总有核细胞计数,在0、72和168 h进行流武CD34~+细胞分析以及甲基纤维素集落检验.实验结果表明:经过7 d的静态共培养,在添加常规剂量遣血生长因子的培养液中,总有核细胞扩增了(15±2.85)倍,CD34~+细胞扩增了(5.33±0.32)倍,CFU-Cs扩增了(5.6±1.21)倍.微胶囊可以作为一种新的共培养隔离手段,微囊化兔骨髓闻充质干细胞在添加适量血清或者造血生长因子组合的条件下对于脐带血造血千/祖细胞在静态下的扩增有明显的促进作用.
장제대혈단개핵세포여포매유토골수간충질간세포적해조산개미효주재3충불동적배양액중진행료7 d적체외정태공배양.매24 h진행총유핵세포계수,재0、72화168 h진행류무CD34~+세포분석이급갑기섬유소집락검험.실험결과표명:경과7 d적정태공배양,재첨가상규제량견혈생장인자적배양액중,총유핵세포확증료(15±2.85)배,CD34~+세포확증료(5.33±0.32)배,CFU-Cs확증료(5.6±1.21)배.미효낭가이작위일충신적공배양격리수단,미낭화토골수문충질간세포재첨가괄량혈청혹자조혈생장인자조합적조건하대우제대혈조혈천/조세포재정태하적확증유명현적촉진작용.
The coculture of cord blood mononuclear cells (CB MNCs) and alginate beads containing rabbit bone marrow (BM) mesenchymal stem cells (MSCs) was investigated in three kinds of culture mediums under the static condition for 7 days. The total nucleated cell density was measured every 24 hours. Flow cytometric assay for CD34~+ cells and methylcellulose colony assays were carried out at 0,72 and 168 h. The results show that the total nucleated cell number is multipled by (15±2.85) times,CD34~+ cells (5.33±0.32) times and colony-forming units in culture (CFU-Cs) (5.6±1.21) times respectively at the end of the 7th day's coculture in the serum-free medium supplemented with conventional dose of cytokines. Microcapsules may be the appropriate coculture protocol and the encapsulated rabbit BM MSCs illustrate remarkable effects on CB hematopoietic stem/progenitor cells expansion when serum or proper cytokines are added under the static condition.