CAJ | 학술논문

目的探讨40例食管癌组织中α型叶酸受体(FOLR1)基因表达及ABO血型相关性。方法收集40例新鲜食管癌，分析其ABO血型;应用免疫组织化学(IHC)及即时定量逆转录PCR检测癌组织及及癌旁组织FRα的表达。结果经IHC检测食管癌组织20％呈(±)，癌旁组织50％呈(±)，10％呈(+)，两者差异有统计学意义(X2=14.48，P＜0.01);Real Time RT-PCR提示癌组织低表达FOLR1，与癌旁组织(F=53.247，P=0.000)、正常食管黏膜组织(F=116.500，P=0.000)相比，差异均有统计学意义(P＜0.01);食管癌患者ABO血型与FOLR1的表达无明显相关性(P=0.647，P＞0.05)。结论食管癌灶与癌旁组织相比FOLR1基因表达降低;检测癌组织、癌旁组织中FRα的表达水平对判断食管癌肿瘤病变范围和指导确定手术治疗范围具有一定意义;ABO血型对FRα在食管癌组织中的表达水平无明显影响。
목적 탐토40례식관암조직중α형협산수체(FOLR1)기인표체급ABO혈형상관성。방법수집40례신선식관암，분석기ABO혈형;응용면역조직화학(IHC)급즉시정량역전록PCR검측암조직급급암방조직FRα적표체。결과경IHC검측식관암조직20％정(±)，암방조직50％정(±)，10％정(+)，량자차이유통계학의의(X2=14.48，P＜0.01);Real Time RT-PCR제시암조직저표체FOLR1，여암방조직(F=53.247，P=0.000)、정상식관점막조직(F=116.500，P=0.000)상비，차이균유통계학의의(P＜0.01);식관암환자ABO혈형여FOLR1적표체무명현상관성(P=0.647，P＞0.05)。결론식관암조여암방조직상비FOLR1기인표체강저;검측암조직、암방조직중FRα적표체수평대판단식관암종류병변범위화지도학정수술치료범위구유일정의의;ABO혈형대FRα재식관암조직중적표체수평무명현영향。
Objective To investigate the expressions of folate receptor alpha (FOLR1) in 40 esophageal cancerous tissues and its relevance of ABO blood group.Methods ABO blood groups were analyzed in 40 patients.Immunohistochemistry (IHC) and real-time quantitative reverse transcription PCR detection were used to detect the expression of folate receptor in cancer and adjacent tissues.Results 20％of esophageal cancer was suspected positive (+), 50％ of cancer adjacent tissue was suspected positive (+), 10％ were positive (+), the difference was statistically significant (x2 = 14.48, P ＜0.01).Real Time RT-PCR analysis showed FOLRI low expression in esophageal cancer and adjacent tissues, compared to normal esophageal mucosa, the differences were statistically significant (F1 =53.247, F2 = 116.500, P ＜0.01).ABO blood type and FOLRi in esophageal cancer patients were not significant correlated (P =0.647, P ＞0.05).Conclusions FOLRI expression was decreased in the esophageal lesions compared with adjacent tissues.Detection of FOLRl expression level in esophageal cancerous tissues and the paired adjacent tissues was helpful for judging the extent of disease and guiding surgery treatment.The FOLRI expression level in esophageal carcinoma tissues had no relationship with ABO blood types.