异丙酚对人心房肌细胞瞬时外向整流钾电流的影响
이병분대인심방기세포순시외향정류갑전류적영향
Effect of propofol on transient outward K~+ current In isolated human atrial myocytes
  • 万方数据
    中华麻醉学杂志 중화마취학잡지
    CHINESE JOURNAL OF ANESTHESIOLOGY
    2009年 10期 885-888 ,共4页

    邝素娟%邓春玉%刘宇斌%饶芳%王春晓%单志新%李晓红%林秋雄%杨敏%吴书林%余细勇

    광소연%산춘옥%류우빈%요방%왕춘효%단지신%리효홍%림추웅%양민%오서림%여세용

    二异丙酚%肌细胞%心脏%钾通道 二異丙酚%肌細胞%心髒%鉀通道
    이이병분%기세포%심장%갑통도
    Propofol%Myecytes%cardiac%potassium channels
    目的 探讨异丙酚对人心房肌细胞瞬时外向整流钾电流(I_(to))的影响.方法 体外循环下行冠状动脉旁路移植术患者,术中于上腔静脉插管前取下右心耳组织,采用急性两步酶解法消化分离成单个心房肌细胞,室温下进行膜片钳全细胞模式记录I_(to).记录不同浓度(25、50、100、150、200 靘ol/L)异丙酚在+50 mV钳制电压下I_(to)的变化,并记录50μmol/L异丙酚对I_(to)激活、失活、失活后再激活动力学的影响.结果 与给药前相比,50、100、150、200 μmol/L异丙酚呈浓度依赖性地抑制I_(to)峰电流密度(P<0.05).给药前、后半数激活电压和斜率因子、半数失活电压和斜率因子、时间常数比较差异无统计学意义(P>0.05).结论 异丙酚可呈浓度依赖性地抑制人心房肌细胞I_(to),产生负性肌力作用,可能是其抗心律失常作用的重要机制之一.
    목적 탐토이병분대인심방기세포순시외향정류갑전류(I_(to))적영향.방법 체외순배하행관상동맥방로이식술환자,술중우상강정맥삽관전취하우심이조직,채용급성량보매해법소화분리성단개심방기세포,실온하진행막편겸전세포모식기록I_(to).기록불동농도(25、50、100、150、200 정ol/L)이병분재+50 mV겸제전압하I_(to)적변화,병기록50μmol/L이병분대I_(to)격활、실활、실활후재격활동역학적영향.결과 여급약전상비,50、100、150、200 μmol/L이병분정농도의뢰성지억제I_(to)봉전류밀도(P<0.05).급약전、후반수격활전압화사솔인자、반수실활전압화사솔인자、시간상수비교차이무통계학의의(P>0.05).결론 이병분가정농도의뢰성지억제인심방기세포I_(to),산생부성기력작용,가능시기항심률실상작용적중요궤제지일.
    Objective To investigate the effect of propofol on transient outward K~+ current (I_(to)) in isolated human atrial myocytes. Methods Atrial myocytes were enzymafically isolated from specimens of human atrial appendages obtained from patients undergoing coronary artery bypass grafting. I_(to) was recorded in voltage-clamp mode using the patch-clamp technique at room temperature. Current signals were recorded with an Axopatch 200B amplifier using the Digidata 1322A-pClamp 9.0 data-acquisltion system. Results Propofol decreased I_(to) in a concentration-dependent manner in human atrial myocytes. The current-voltnge curve was significantly decreased. Propofol(25,52,100,150 and 200 μmol/L) decreased I_(to) current density (9.89±0.03)%,(52.38±0.14)%,(61.75±0.12)%,(71.30±0.13)% and (81.31±0.11)% respectively.The I_(10) activation curve,the inactivation curve and the recovery curve were not significantly affected. Conclusion Propefol significantly decreases I_(to) in human atrial myocytes in concentration-manners.
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