CAJ | 학술논문

目的探讨局部温热对小鼠接触性超敏反应激发部位朗格汉斯细胞(LC)数目和形态的影响.方法 8周龄雌性SPF级Balb/c小鼠60只,随机分为致敏前3 d加热组、致敏同时加热组和致敏2 d后加热组,每组20只.分别于致敏前3 d、致敏同时和致敏2 d后在背部致敏部位施以37℃、39℃、41℃、43℃的局部温热(各5只)20 min.同时设立5只致敏一不加热组作为对照.于小鼠右耳背侧激发后2 d,分离右耳背侧表皮,采用免疫组化方法检测表皮LC的数目和形态变化.结果随所施温度的升高,LC数目在致敏前加热组逐渐减少,37℃、39℃、41℃、43℃依次为(321.83±41.81)、(251.12±16.29)、(191.41±28.7)、(128.33±77.61)个/mm2,组间差异有统计学意义(P<0.05);在致敏同时加热组逐渐增多,但差异无统计学意义(P>0.05);在致敏后加热组也逐渐增多,依次为(320.83±113.6)、(398.33±31.91)、(437.83±29.78)、(477.25±86.79)个/mmz,差异有统计学意义(P<0.01).无论致敏前3 d、致敏同时和致敏2 d后施以不同温热条件,LC的树突随所施温度升高呈增多、增长的趋势;但在43℃条件下,其趋势略有下降.结论致敏部位的局部温热对激发部位LC形态和密度有影响,可能与接触性超敏反应的严重程度有关.
목적 탐토국부온열대소서접촉성초민반응격발부위랑격한사세포(LC)수목화형태적영향.방법 8주령자성SPF급Balb/c소서60지,수궤분위치민전3 d가열조、치민동시가열조화치민2 d후가열조,매조20지.분별우치민전3 d、치민동시화치민2 d후재배부치민부위시이37℃、39℃、41℃、43℃적국부온열(각5지)20 min.동시설립5지치민일불가열조작위대조.우소서우이배측격발후2 d,분리우이배측표피,채용면역조화방법검측표피LC적수목화형태변화.결과 수소시온도적승고,LC수목재치민전가열조축점감소,37℃、39℃、41℃、43℃의차위(321.83±41.81)、(251.12±16.29)、(191.41±28.7)、(128.33±77.61)개/mm2,조간차이유통계학의의(P<0.05);재치민동시가열조축점증다,단차이무통계학의의(P>0.05);재치민후가열조야축점증다,의차위(320.83±113.6)、(398.33±31.91)、(437.83±29.78)、(477.25±86.79)개/mmz,차이유통계학의의(P<0.01).무론치민전3 d、치민동시화치민2 d후시이불동온열조건,LC적수돌수소시온도승고정증다、증장적추세;단재43℃조건하,기추세략유하강.결론 치민부위적국부온열대격발부위LC형태화밀도유영향,가능여접촉성초민반응적엄중정도유관.
Objective To investigate the effect of local hyperthermia on the morphology and quantity of Langerhans cells (LCs)at challenged skin sites of a mouse model of contact hypersensitivity.Methods Sixty mice were equally divided into 3 groups to be treated with local hyperthermia (37℃,39℃,41℃and 43℃)for 20 minutes at sensitization sites on the back of mice 3 days before (pre-heat group),concurrently with (concurrent-heat group)or 2 days after(post-heat group)sensitization respectively.Five mice which remained unsensitized and untreated served as the controls.Five days after the sensitization,the mice were challenged on the dorsal surface of right ears.Two days after the elicitation,the right ears were resected and immunohistochemistry was performed to observe the morphology and determine the quantity of LCs at challenged sites.Results With the rise in temperature,the number of LCs in the epidermis of ear skin decreased in pre-heat group(321.83±41.81,251.12±16.29,191.41±28.7,128.33±77.61 per square millimeter at 37 ℃,39 ℃,41℃and 43℃,respectively,P<0.05),but increased gradually in the concurrent-heat group(309.08±84.69,355.33±11.38,405.5±55.25,438.16±99.56 per square millimeter at 37℃,39℃,41℃ and 43℃,respectively,P>0.05)and in the post-heat group(320.83±113.6,398.33±31.91,437.83±29.78,477.25±86.79 per square millimeter at 37℃,39 ℃,41℃and 43℃,respectively,P<0.01).The dendrites of LCs increased in number and length when the temperature lose from 37 ℃ to 41℃,but slightly declined at 43℃.Conclusions Local hyperthermia at sensitization sites could affect the morphology and density of LCs at challenged sites,and the effect is likely associated with the severity of contact hypersensitivity.