CAJ | 학술논문

目的研究MNA细胞培养法检测和分离狂犬病街毒的可行性.方法用MNA细胞培养法、荧光抗体实验(FAT)和双抗体夹心ELISA方法分别检测33份狂犬病街毒样品、20份狂犬病毒阴性犬脑样品和4份正常鼠脑样品.结果 MNA细胞培养法检测57份待检样品,培养48 h的实验结果显示,33份狂犬病街毒样品均为阳性,狂犬病毒阴性犬脑样品和正常鼠脑样品均为阴性,与FAT、双抗体夹心ELISA方法的检测结果完全一致.结论 MNA细胞培养法对样品中狂犬病街毒的检测具有较高的灵敏度和特异性,可用于狂犬病街毒的检测和分离.
목적 연구MNA세포배양법검측화분리광견병가독적가행성.방법 용MNA세포배양법、형광항체실험(FAT)화쌍항체협심ELISA방법분별검측33빈광견병가독양품、20빈광견병독음성견뇌양품화4빈정상서뇌양품.결과 MNA세포배양법검측57빈대검양품,배양48 h적실험결과현시,33빈광견병가독양품균위양성,광견병독음성견뇌양품화정상서뇌양품균위음성,여FAT、쌍항체협심ELISA방법적검측결과완전일치.결론 MNA세포배양법대양품중광견병가독적검측구유교고적령민도화특이성,가용우광견병가독적검측화분리.
Objective Feasibility of using MNA cell-culture inoculation test to detect and isolate the street rabies virus. Methods Using MNA cell-culture inoculation test, fluorescent antibody test (FAT) and sandwich ELISA with double-antibodies to detect 33 specimens of street rabies virus, 20 specimens of negative canine brains and 4 specimens of healthy mice brains. Results 33 specimens of street rabies virus were positive to the cell-culture inoculation test but the others were negative. The concordances of MNA cell-cultured inoculation test with FAT and sandwich ELISA with double-antibodies were both 100%. Conclusion MNA cell-culture inoculation test appeared to be both highly sensitive and specific in detecting the street rabies virus, and could be used in detection and isolation of the virus.