中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2011年
11期
1110-1114
,共5页
胶质母细胞瘤%阿司匹林%放射敏感性
膠質母細胞瘤%阿司匹林%放射敏感性
효질모세포류%아사필림%방사민감성
Glioblastomamultiforme%Aspirin%Radiosensitivity
目的 观察阿司匹林(ASA)对人胶质母细胞瘤U87细胞系放射敏感性的影响并探讨其可能机制.方法 常规培养U87细胞,CCK-8法检测16、8、4、2、1、0.5 mmol/L ASA对细胞抑制率和1 mmol/L ASA预处理对2、4、8 Gy 6MV-X射线单次照射后细胞抑制率的影响,计算ASA对这3种剂量射线的放射增敏比；选取1 mmol/L ASA和8 Gy照射剂量进行实验,分为对照组、照射组、ASA组、ASA+照射组,流式细胞仪检测细胞凋亡和NF-κB的含量,激光共聚焦显微镜观察NF-κB的表达.结果 与空白对照组比较,0.5、1 mmol/L ASA组细胞抑制率的差异无统计学意义(P＞0.05)；ASA对8Gy射线的放射增敏比高于2、4Gy,差异有统计学意义(P＜0.05).流式细胞仪检测结果显示,与照射组比较,ASA+照射组细胞凋亡率较高,NF-κB含量较低,差异均有统计学意义(P＜0.05).激光共聚焦显微镜下观察显示,照射组细胞NF-κB在细胞质、细胞核均有表达,与ASA+照射组细胞比较表达较强.结论 ASA可提高胶质母细胞瘤的放射敏感性,它可能通过抑制NF-κB的表达而增加由射线介导的胶质瘤细胞的凋亡,从而增强细胞对射线的反应.
目的 觀察阿司匹林(ASA)對人膠質母細胞瘤U87細胞繫放射敏感性的影響併探討其可能機製.方法 常規培養U87細胞,CCK-8法檢測16、8、4、2、1、0.5 mmol/L ASA對細胞抑製率和1 mmol/L ASA預處理對2、4、8 Gy 6MV-X射線單次照射後細胞抑製率的影響,計算ASA對這3種劑量射線的放射增敏比；選取1 mmol/L ASA和8 Gy照射劑量進行實驗,分為對照組、照射組、ASA組、ASA+照射組,流式細胞儀檢測細胞凋亡和NF-κB的含量,激光共聚焦顯微鏡觀察NF-κB的錶達.結果 與空白對照組比較,0.5、1 mmol/L ASA組細胞抑製率的差異無統計學意義(P＞0.05)；ASA對8Gy射線的放射增敏比高于2、4Gy,差異有統計學意義(P＜0.05).流式細胞儀檢測結果顯示,與照射組比較,ASA+照射組細胞凋亡率較高,NF-κB含量較低,差異均有統計學意義(P＜0.05).激光共聚焦顯微鏡下觀察顯示,照射組細胞NF-κB在細胞質、細胞覈均有錶達,與ASA+照射組細胞比較錶達較彊.結論 ASA可提高膠質母細胞瘤的放射敏感性,它可能通過抑製NF-κB的錶達而增加由射線介導的膠質瘤細胞的凋亡,從而增彊細胞對射線的反應.
목적 관찰아사필림(ASA)대인효질모세포류U87세포계방사민감성적영향병탐토기가능궤제.방법 상규배양U87세포,CCK-8법검측16、8、4、2、1、0.5 mmol/L ASA대세포억제솔화1 mmol/L ASA예처리대2、4、8 Gy 6MV-X사선단차조사후세포억제솔적영향,계산ASA대저3충제량사선적방사증민비；선취1 mmol/L ASA화8 Gy조사제량진행실험,분위대조조、조사조、ASA조、ASA+조사조,류식세포의검측세포조망화NF-κB적함량,격광공취초현미경관찰NF-κB적표체.결과 여공백대조조비교,0.5、1 mmol/L ASA조세포억제솔적차이무통계학의의(P＞0.05)；ASA대8Gy사선적방사증민비고우2、4Gy,차이유통계학의의(P＜0.05).류식세포의검측결과현시,여조사조비교,ASA+조사조세포조망솔교고,NF-κB함량교저,차이균유통계학의의(P＜0.05).격광공취초현미경하관찰현시,조사조세포NF-κB재세포질、세포핵균유표체,여ASA+조사조세포비교표체교강.결론 ASA가제고효질모세포류적방사민감성,타가능통과억제NF-κB적표체이증가유사선개도적효질류세포적조망,종이증강세포대사선적반응.
Objective To observe the effect of aspirin(ASA)on radiosensitivity of human glioblastoma multiforme cell line U87,and explore its mechanism.Methods Routine culture of U87cell line was performed; inhibitory effect of different doses of ASA(16,8,4,2,1 and 0.5 mmol/L)on the proliferation of U87 cells and the influence of 1 mmol/L ASA pretreatment on the proliferation of U87cells after single fraction irradiation with 2,4 and 8 Gy 6MV-X ray were detected with CCK-8; the radiation enhancement ratio(RER)of ASA on these 3 different radiation doses were calculated.We chose 1 mmol/L ASA and 8 Gy X-ray for further experiment; the U87 cells were divided into control group,radiation treatment group,ASA treatment group and radiation plus ASA treatment group; the changes of apoptosis rate and the level of NF-κB in each group were detected by flow cytometry(FCM),and the expression of NF-κB was observed by laser scanning confocal microscope.Results As compared with that of control group(not given ASA),the inhibitory effect on proliferation of U87 cells in 1 and 0.5mmol/L ASA treatment groups was not significantly different(P＞0.05).The RER in radiation dose of 2,4and 8 Gy induced by ASA was(0.155±0.008),(0.205±0.017)and(0.392±0.024),respectively; 8 Gy treatment group had a significantly higher RER than 2 and 4 Gy treatment groups(P＜0.05).FCM showed that ASA plus 8 Gy radiation treatment could increase the apoptosis rate of U87 from(7.74％±0.43％)to (12.58％±0.94％),however,it could decrease the level of NF-κB in U87 cells from(96.65％±2.79％)to (77.06％.±2.89％); significant differences between control group and ASA plus radiation treatment group were noted(P＜0.05).Laser scanning confocal microscope showed that the expression of NF-κB mainly appeared in the cytoplasm ad nucleus after irradiation,and its expression could be inhibited by ASA.Conclusion ASA could increase the radiosensitivity of U87 cells by inhibiting the expression of NF-κB to increase the apoptosis of glioma cells.
