CAJ | 학술논문

目的探讨新型甲型流感病毒(2009H1N1)血凝素(HA)DNA疫苗诱导小鼠产生中和抗体特性.方法构建2009H1N1或1918甲型流感病毒(1918H1N1)HA蛋白表达质粒2009HA和1918HA,采用25μg或200μg剂量2009HA质粒免疫小鼠,以2009HA或1918HA蛋白为包被抗原,测定小鼠血清中2009HA抗体总量或交叉反应抗体含量,分别用2009H1N1和1918H1N1两种假病毒(pp)测定抗体中和活性.结果 25 μg或200μg的2009HA质粒加强免疫小鼠后,4～16周内两组小鼠血清中2009HA总抗体水平以及对2009H1N1pp的中和抗体滴度相似(P>0.05),都含有与1918HA蛋白交叉反应抗体,对1918H1N1pp的交叉中和抗体滴度相似(P>0.05).结论小剂量2009HA质粒DNA疫苗能够诱导小鼠产生持久的高水平中和抗体,对于预防新现流感病毒具有潜在应用价值.
목적 탐토신형갑형류감병독(2009H1N1)혈응소(HA)DNA역묘유도소서산생중화항체특성.방법 구건2009H1N1혹1918갑형류감병독(1918H1N1)HA단백표체질립2009HA화1918HA,채용25μg혹200μg제량2009HA질립면역소서,이2009HA혹1918HA단백위포피항원,측정소서혈청중2009HA항체총량혹교차반응항체함량,분별용2009H1N1화1918H1N1량충가병독(pp)측정항체중화활성.결과 25 μg혹200μg적2009HA질립가강면역소서후,4～16주내량조소서혈청중2009HA총항체수평이급대2009H1N1pp적중화항체적도상사(P>0.05),도함유여1918HA단백교차반응항체,대1918H1N1pp적교차중화항체적도상사(P>0.05).결론 소제량2009HA질립DNA역묘능구유도소서산생지구적고수평중화항체,대우예방신현류감병독구유잠재응용개치.
Objective To study the characteristics of neutralization antibody in mice induced by DNA vaccines of hemagglutinin(HA) of novel H1N1 influenza A virus(2009H1N1).Methods HA encoding plasmids of 2009H1N1 or 1918H1N1(2009HA or 1918HA)were constructed.25 μg or 200 μg dosage of 2009HA plasmids were used to immunize the mice,the total antibody of anti-20O9HA or cross-reactive antibody were assayed by ELISA using 2009HA or 1918HA protein as capture antigen,and the neutralizing antibody were assayed by two kinds of virus pseudo - particles(pp) of 2009H1N1 and 1918H1N1 .Results During of 4 to 16 weeks after boost immunization,in two groups of mice immunized with 25 μg or 200 μg dosage 2009HA plasmids,both total antibody of anti-2009HA and neutralizing antibody to 2009H1Nlpp reached the similar level(P ＞0.05),and there were cross-reactive antibody to 1918HA protein in two groups of mice serum,with similar titers of cross-neutralizing activity to 1918H1N1 pp(P ＞0.05),Conclusion A low dosage DNA vaccine encoding HA of 2009 H1 N1 virus is able to induce persistent and high level of neutralizing antibody,and may be potential valuable vaccine against the new emerging influenza virus.