CAJ | 학술논문

目的研究柯萨奇病毒腺病毒受体(CAR)的mRNA和蛋白在正常肺组织、癌旁组织及肺癌组织中的表达情况,分析其与肺癌发生发展的相关性及在不同临床病理参数间的表达差异.方法应用半定量逆转录聚合酶链反应(RT-PCR)方法和Western blot检测正常肺组织、癌旁组织和肺癌组织中CAR mRNA和CAR蛋白的表达情况.结果 CAR mRNA和蛋白在正常肺组织、癌旁组织和肺癌组织中的表达最分别为1.000±0.012、1.048±0.035、1.282±0.072和0.902±0.038、0.944±0.042、1.08±0.052,其差异均有统计学意义(P<0.05).CAR mRNA和CAR蛋白在肺癌中的表达呈正相关(r=0.448,P=0.026).在不同的临床病理参数之间,CAR mRNA和蛋白的表达在患者性别、年龄、吸烟与否、肿瘤大小、有无淋巴结转移及TNM分期中差异无统计学意义(P>0.05);CAR的表达在不同的病理类型中差异具有统计学意义(P=0.012),在7例细支气管肺泡痛(BAC)中均高表达(P=0.029).结论肺癌组织中CAR的表达水平显著高于正常肺组织和癌旁组织,且在不同病理类型的表达上存在差异,提示CAR在肺癌的发生发展过程中起到了重要作用,并可能成为潜在的肿瘤标记及预后指标.
목적 연구가살기병독선병독수체(CAR)적mRNA화단백재정상폐조직、암방조직급폐암조직중적표체정황,분석기여폐암발생발전적상관성급재불동림상병리삼수간적표체차이.방법 응용반정량역전록취합매련반응(RT-PCR)방법화Western blot검측정상폐조직、암방조직화폐암조직중CAR mRNA화CAR단백적표체정황.결과 CAR mRNA화단백재정상폐조직、암방조직화폐암조직중적표체최분별위1.000±0.012、1.048±0.035、1.282±0.072화0.902±0.038、0.944±0.042、1.08±0.052,기차이균유통계학의의(P<0.05).CAR mRNA화CAR단백재폐암중적표체정정상관(r=0.448,P=0.026).재불동적림상병리삼수지간,CAR mRNA화단백적표체재환자성별、년령、흡연여부、종류대소、유무림파결전이급TNM분기중차이무통계학의의(P>0.05);CAR적표체재불동적병리류형중차이구유통계학의의(P=0.012),재7례세지기관폐포통(BAC)중균고표체(P=0.029).결론 폐암조직중CAR적표체수평현저고우정상폐조직화암방조직,차재불동병리류형적표체상존재차이,제시CAR재폐암적발생발전과정중기도료중요작용,병가능성위잠재적종류표기급예후지표.
Objective To investigate the mRNA and proten expression of coxsackievirus and adenovirns receptor (CAR) in the corresponding normal lung tissue, para-neoplastic tissue and lung cancer tissue,and the correlation of CAR expression with the carcinogenesis as well as the expression difference in various clinicopathologic parameters. Methods The expression of CAR mRNA and protein in the samples from 32 lung cancer patients was determined by RT-PCR and Western blot, respectively. Results The expression level of CAR mRNA and protein in normal lung tissue, paraneoplastic tissue and cancer tissue were 1.000±0.012,1.048±0.035,1.282±0.072,and 0.902±0.038,0.944±0.042,1.08±0.052, respectively, with a statistical significance among the groups (P=0.022, P=0.007, P=0.009, P=0.027). There was a statistically significant positive correlation between expression of CAR mRNA and that of CAR protein (r=0.448, P=0.026). The expression levels of CAR were significantly different among different pathological types(P=0.012), with a high level of CAR in all 7 bronchiolo-alveolar carcinoma (BAC, P=0.029). However, there was no statistical significance in other clinieopathologic parameters (P>0.05), including gender, age, smoking or not, tumor size,with or without lymph node metastasis and TNM stage. Conclusion The expression of CAR mRNA and protein in cancer tissue samples are significantly higher than that in the normal and paraneoplastic samples, indicating that CAR might play a crucial role in the carcinogenesis. It may become a new potential prognostic marker for lung cancer patients.