CAJ | 학술논문

普通小麦(Triticum aestivum L.)材料Qz180具有良好的抗条锈病特性,经基因推导发现其含有一个优良的抗条锈病的基因,暂定名为YrQz.用Qz180与感病材料铭贤169和WL1分别杂交构建了两个F2群体,用条中30号条锈菌小种对这两个群体进行的抗性测验表明,YrQz为显性单基因遗传.通过SSR和AFLP结合BSA的方法对这个基因进行了分子作图,结果鉴定出与YrQz连锁的2个SSR标记和2个AFLP标记.根据SSR标记的染色体位置,该基因被定位在2B染色体的长臂上,位于两个SSR位点Xgwm388和Xgwm526之间;两个AFLP标记P35M48(452)和P36M61(163)分别位于该基因的两侧,遗传距离分别为3.4 cM和4.1cM.
보통소맥(Triticum aestivum L.)재료Qz180구유량호적항조수병특성,경기인추도발현기함유일개우량적항조수병적기인,잠정명위YrQz.용Qz180여감병재료명현169화WL1분별잡교구건료량개F2군체,용조중30호조수균소충대저량개군체진행적항성측험표명,YrQz위현성단기인유전.통과SSR화AFLP결합BSA적방법대저개기인진행료분자작도,결과감정출여YrQz련쇄적2개SSR표기화2개AFLP표기.근거SSR표기적염색체위치,해기인피정위재2B염색체적장비상,위우량개SSR위점Xgwm388화Xgwm526지간;량개AFLP표기P35M48(452)화P36M61(163)분별위우해기인적량측,유전거리분별위3.4 cM화4.1cM.
YrQz, a stripe rust disease resistance gene was identified in a common wheat (Triticum aestivum L.) line Qz180. This resistance was controlled by a single dominant gene, which was confirmed by genetic analysis of two F2 populations derived from the crosses using Qz180 and two susceptible parents (Mingxian 169 and WL1). Bulked segregant analysis using simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) markers was conducted in order to map the chromosomal location of YrQz. The results indicated that YrQz was located on the long arm of wheat chromosome 2B and resided in a region flanked by two SSR loci Xgwm388and Xgwm526. Two AFLP markers P35M48(452) and P36M61 (163) were closely linked to YrQz with the genetic distance of 3.4 cM and 4.1 cM, respectively. To our knowledge, this is the first molecularly mapped stripe rust resistance gene on wheat chromosome 2B.