CAJ | 학술논문

目的探讨体外培养的人牙周膜成纤维细胞(HPDLF)在动态力学微应变范围内细胞增殖和功能活性的变化.方法采用四点弯曲细胞力学加载装置对体外培养的HPDLF进行加载,通过流式细胞术(FCM)分析细胞周期的改变和定量分析细胞总蛋白含量和碱性磷酸酶(ALP)活性,研究不同作用方式、大小和时间的动态力学应变对细胞增殖和功能活性的影响.结果加载装置所产生的动态力学微应变范围对HPDLF的G_0/G_1期、S期细胞百分比、增殖指数PI、总蛋白含量及ALP活性产生明显的影响(P<0.05),其中G_0/G_1期细胞百分比减少,S期细胞百分比、PI、总蛋白含量及ALP活性增高,且此影响均与应变的大小和作用时间密切相关;动态力学应变加载方式的不同对HPDLF的G_0/G_1期、S期、G_2/M期细胞百分比、PI、总蛋白含量及ALP活性均有明显的影响(P<0.05).梯度加载组的促增殖和对总蛋白含量及ALP活性的上调作用均明显高于1000、4000 μstrain两组.结论HPDLF细胞增殖和功能活性增加与动态力学应变的作用方式、应变大小、作用时间密切相关.
목적 탐토체외배양적인아주막성섬유세포(HPDLF)재동태역학미응변범위내세포증식화공능활성적변화.방법 채용사점만곡세포역학가재장치대체외배양적HPDLF진행가재,통과류식세포술(FCM)분석세포주기적개변화정량분석세포총단백함량화감성린산매(ALP)활성,연구불동작용방식、대소화시간적동태역학응변대세포증식화공능활성적영향.결과 가재장치소산생적동태역학미응변범위대HPDLF적G_0/G_1기、S기세포백분비、증식지수PI、총단백함량급ALP활성산생명현적영향(P<0.05),기중G_0/G_1기세포백분비감소,S기세포백분비、PI、총단백함량급ALP활성증고,차차영향균여응변적대소화작용시간밀절상관;동태역학응변가재방식적불동대HPDLF적G_0/G_1기、S기、G_2/M기세포백분비、PI、총단백함량급ALP활성균유명현적영향(P<0.05).제도가재조적촉증식화대총단백함량급ALP활성적상조작용균명현고우1000、4000 μstrain량조.결론HPDLF세포증식화공능활성증가여동태역학응변적작용방식、응변대소、작용시간밀절상관.
Objective To study the changes in the proliferation and synthetic function of human periodontal ligament fibroblasts (HPDLF) in response to dynamic mechanical strains of different modes, magnitudes and durations. Methods Using a 4-point bending system, the effect of dynamic mechanical strains of different modes, magnitudes and durations on the proliferation of HPDLF was investigated by analyzing the cell cycle changes with flow cytometry (FCM), and the total protein level and the activity of alkaline phosphatase (ALP) in HPDLF were assayed by quantitative analysis. Results The percentage of G_0/G_1 cell decreased, S phase cells increased, and the proliferation index (PI), total protein level and activity of ALP were augmented significantly in response to dynamic mechanical micro-strains. These changes showed close eorrelatious to the magnitude and duration of the strain. The mode of strain caused significant changes in G_0/G_1, S, and G_2/M phase cell percentages as well as the PI, total protein level and ALP activity of the cells. In the gradient strain group, the cell proliferation activity, total protein level and ALP activity were obviously higher than those in 1000 and 4000 μstrain groups. Conclusion The changes in the proliferation and synthetic function of HPDLF are closely correlated to the mode, magnitude and duration of the strains.