CAJ | 학술논문

通过在土壤中反复添加不同浓度的土霉素进行预处理.采用平板稀释法测定了土壤细菌在含土霉素培养基及普通培养基上的生长情况.用耐药菌发生率作为指标评价了土霉素污染浓度和污染次数对土壤抗土霉素可培养细菌的影响.结果表明,土霉素污染可诱导土壤土霉素抗性细菌的产生,耐药菌发生率与土霉素添加浓度和添加次数有关.当土霉素添加浓度大于4 mg/kg时,一次处理就可诱导土壤可培养细菌产生耐药性;当土霉索浓度为3 mg/kg时,二次处理可诱导土壤可培养细菌产生耐药性;当土霉索浓度为2 mg/kg时,需要5次处理才可诱导土壤可培养细菌产生耐药性;而当土霉索浓度为1 mg/kg时,5次处理均没有明显诱导土壤可培养细菌产生耐药性.研究结果表明.长期施用含土霉素的畜禽粪可导致土壤土霉素抗性细菌的形成和发展.
통과재토양중반복첨가불동농도적토매소진행예처리.채용평판희석법측정료토양세균재함토매소배양기급보통배양기상적생장정황.용내약균발생솔작위지표평개료토매소오염농도화오염차수대토양항토매소가배양세균적영향.결과표명,토매소오염가유도토양토매소항성세균적산생,내약균발생솔여토매소첨가농도화첨가차수유관.당토매소첨가농도대우4 mg/kg시,일차처리취가유도토양가배양세균산생내약성;당토매색농도위3 mg/kg시,이차처리가유도토양가배양세균산생내약성;당토매색농도위2 mg/kg시,수요5차처리재가유도토양가배양세균산생내약성;이당토매색농도위1 mg/kg시,5차처리균몰유명현유도토양가배양세균산생내약성.연구결과표명.장기시용함토매소적축금분가도치토양토매소항성세균적형성화발전.
Antibiotics enter agricultural soils by the use of animal excrements as fertilizers.However.it is scarcely known how these antibiotics affect the culltivable soil bacteria antibiotic resistance.Therefore,effect of antibiotic oxytetracycline (OTC) pollution on cultivable soil bacteria antibiotic resistance was studied by addition of different dose of OTC in the soil.Percentage of soil bacteria antibiotic resistance was evaluated by comparing cultivable bacteria number in culture medium spiked with or without OTC.The results showed that soil OTC pollution could increase the cultivable soil bacteria,which Was related to the additional dose and times of OTC.The cuhivable soil bacteria antibiotic resistance was significantly increased when soil was treated once with OTC at the dose of 4 mg·kg~(-1),or repeatedly treated twice at the dose of 3 mg·kg~(-1),or repeatedly treated five times at the dose of 2 mg·kg~(-1).But,no significant increase in the cultivable soil bacteria antibiotic resistance was found when soil was treated with OTC at the dose of 1 mg·kg~(-1).The results suggested that long-term application of antibiotics-containing animal manure could improve the development bf soil bacteria antibiotic resistance.