中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2010年
9期
910-914
,共5页
阿尔茨海默病%β 淀粉样肽%脑灵汤%iNOS%大鼠
阿爾茨海默病%β 澱粉樣肽%腦靈湯%iNOS%大鼠
아이자해묵병%β 정분양태%뇌령탕%iNOS%대서
Alzheimer's disease%β-amyloid polypeptide%Naoling decoction%iNOS%rat
目的:探讨脑灵汤对阿尔茨海默病(AD)模型大鼠海马CA3区iNOS蛋白表达的影响,初步揭示脑灵汤可能的作用机制.方法:选取40只SPF清洁级SD大鼠,随机分为正常对照组、假手术组、AD组、脑灵汤组和脑复康组,每组8只,喂养1周后,采用Aβ1-42注射大鼠海马法制成大鼠模型,再喂养28 d,免疫组织化学法观察iNOS蛋白在大鼠海马CA3区中的表达.结果:AD组大鼠海马CA3区iNOS蛋白表达较正常对照组增多(P<0.05),脑灵汤治疗后iNOS蛋白的表达降低. 结论:脑灵汤能明显改善AD模型海马CA3区iNOS表达,对老年性痴呆大鼠有治疗作用.
目的:探討腦靈湯對阿爾茨海默病(AD)模型大鼠海馬CA3區iNOS蛋白錶達的影響,初步揭示腦靈湯可能的作用機製.方法:選取40隻SPF清潔級SD大鼠,隨機分為正常對照組、假手術組、AD組、腦靈湯組和腦複康組,每組8隻,餵養1週後,採用Aβ1-42註射大鼠海馬法製成大鼠模型,再餵養28 d,免疫組織化學法觀察iNOS蛋白在大鼠海馬CA3區中的錶達.結果:AD組大鼠海馬CA3區iNOS蛋白錶達較正常對照組增多(P<0.05),腦靈湯治療後iNOS蛋白的錶達降低. 結論:腦靈湯能明顯改善AD模型海馬CA3區iNOS錶達,對老年性癡呆大鼠有治療作用.
목적:탐토뇌령탕대아이자해묵병(AD)모형대서해마CA3구iNOS단백표체적영향,초보게시뇌령탕가능적작용궤제.방법:선취40지SPF청길급SD대서,수궤분위정상대조조、가수술조、AD조、뇌령탕조화뇌복강조,매조8지,위양1주후,채용Aβ1-42주사대서해마법제성대서모형,재위양28 d,면역조직화학법관찰iNOS단백재대서해마CA3구중적표체.결과:AD조대서해마CA3구iNOS단백표체교정상대조조증다(P<0.05),뇌령탕치료후iNOS단백적표체강저. 결론:뇌령탕능명현개선AD모형해마CA3구iNOS표체,대노년성치태대서유치료작용.
Objective To observe the effect of Naoling decoction on the expression of iNOS in hippocamal CA3 region in rats with Alzheimer's disease (AD), and to explore the potential mechanism. Methods Forty SD rats were classified into 5 groups: a normal group, a sham operated group, an AD group, a Naoling decoction group, and a Naofukang group. The Alzheimer's disease model was established by injecting Aβ1-42 into the hippocamal in the rats. Expression of iNOS in the CA3 region was measured with immunohistochemical staining. Results Compared with the normal group, the expression of iNOS protein in the hippocampal CA3 fields in the AD group increased (P<0.05). Naoling decoction decreased the iNOS expression. Conclusion Naoling decoction can significantly reduce the expression of iNOS in the AD model rats, suggesting that Naoling decoction can be used for AD rats.
