CAJ | 학술논문

目的研究胰岛β细胞损伤后胰岛再生过程中Wnt/β-catenin信号通路的生物调控.方法:STZ破坏新生大鼠胰岛β细胞,免疫组织化学观察1d,2d,3d中Wnt/βcatenin通路βcatenin为核心的上、下游基因APC,c-Myc以及Nestin在胰岛再生过程中的表达.结果:β-catenin在残留的胰岛细胞中表达上调,并在胰腺间质中异常表达;APC表达于胰腺导管上皮及残留的胰岛细胞;c-My.则主要表达于胰腺腺泡上皮及胰腺间质内;Nestin表达分布于残留的胰岛细胞和胰腺导管上皮.结论:Wnt/β-catenin信号通路在胰岛再生中可能通过促进胰腺干细胞分化与增殖而调控.
목적 연구이도β세포손상후이도재생과정중Wnt/β-catenin신호통로적생물조공.방법:STZ파배신생대서이도β세포,면역조직화학관찰1d,2d,3d중Wnt/βcatenin통로βcatenin위핵심적상、하유기인APC,c-Myc이급Nestin재이도재생과정중적표체.결과:β-catenin재잔류적이도세포중표체상조,병재이선간질중이상표체;APC표체우이선도관상피급잔류적이도세포;c-My.칙주요표체우이선선포상피급이선간질내;Nestin표체분포우잔류적이도세포화이선도관상피.결론:Wnt/β-catenin신호통로재이도재생중가능통과촉진이선간세포분화여증식이조공.
Objective To investigate the regulation role of Wnt/β-catenin signal pathway in regulating pancreatic islets regeneration after β-cells injury.MethodSTZ were used to injure β-cells and imunohistochemistry method was adopted to check the expression of several vital factors e.g. APC, β-catenin, c-Myc for Wnt/β-catenin signal pathway and of nestin in pancreatic islets regeneration. Results Immunohistochemistry showed that the β-catenin was up-regulated in β-cells and abnormally expressed in pancreatic stroma; APC was expressed on duct epithelium and β cells; C-myc was expressed in stroma and gland alveolus epithelium; nestin was expressed on islet cell and duct epithelium.ConclusionThe regulation roles of Wnt/β-catenin signal pathway in pancreatic islets regeneration may be through promoting pancreatic stem cells differentiation and proliferation. 　