CAJ | 학술논문

目的研究骨髓间充质干细胞(Bone Marrow Mesenchymal Stem Cells,BMSCs)及其分化的神经样细胞分别与聚乳酸-羟基乙酸(polylactic glycolic acid,PLGA)支架材料复合修复大鼠神经损伤的效果.方法将BMSCs复合PIGA培养,通过扫描电镜观察BMSCs在PLGA上的黏附、增殖并诱导分化成神经样细胞的情况;然后分别将单纯的PLGA、BMSCs-PLGA复合物以及神经样细胞-PLGA复合物分别移植入3组右侧坐骨神经损伤的SD大鼠模型中,术后观察三组动物60 d.结果 BMSCs及诱导分化生成的神经样细胞能在PLGA良好的黏附、增殖和生长;复合PLGA移植后能修复损伤的神经.结论 BMSCs及神经样细胞复合PLGA移植可作为组织工程治疗脊髓损伤及糖尿病晚期神经源性膀胱的一种方法.
목적 연구골수간충질간세포(Bone Marrow Mesenchymal Stem Cells,BMSCs)급기분화적신경양세포분별여취유산-간기을산(polylactic glycolic acid,PLGA)지가재료복합수복대서신경손상적효과.방법 장BMSCs복합PIGA배양,통과소묘전경관찰BMSCs재PLGA상적점부、증식병유도분화성신경양세포적정황;연후분별장단순적PLGA、BMSCs-PLGA복합물이급신경양세포-PLGA복합물분별이식입3조우측좌골신경손상적SD대서모형중,술후관찰삼조동물60 d.결과 BMSCs급유도분화생성적신경양세포능재PLGA량호적점부、증식화생장;복합PLGA이식후능수복손상적신경.결론 BMSCs급신경양세포복합PLGA이식가작위조직공정치료척수손상급당뇨병만기신경원성방광적일충방법.
Objectives To study separately the effect of bone marrow mesenchymal stem cells (BMSCs) and differentiated neural - like cells combined with polylactic glycolic acid ( PLGA ) scaffolds, both composite which restore to SD rat nerve injury. Methods BMSCs were cultured by differential adherence,and composited PLGA,via scanning electron microscope to investigate the information of BMSCs'adhesion, proliferation and induction into neural - like cells in the scaffolds. Bold scaffold, BMSCs - PLGA complex, and neural - like cells - PLGA complex were separately transplanted into SD rat models, with the injury for right sciatic nerve, and obsvered SD rat models during 60 days after operation. Results BMSCs and neural - like cells can be exactly adhere to, proliferate and grow on the PLGA;BMSCs and neural -like cells combined with PLGA, which can restore to nerve injury. Conclusions BMSCs and differentiated neural - like cells combined with polylactic glycolic acid (PLGA) scaffolds,which can be used as a method of tissue engineering,to treat neurogenic bladder caused by spinal cord injury and advanced diabetes.