CAJ | 학술논문

目的用含有金葡菌细胞膜抗原决定簇20肽的麦芽糖结合蛋白免疫Balb/c小鼠,重复并改进本研究小组前人已成功建立的IgAN动物模型.检测分析模型动物的冰冻肾组织切片上的炎症因子巨噬细胞移动抑制因子(MIF)和环氧化酶2(COX-2)的表达及其观察其相关性.方法 ①隔周用含有20肽的麦芽糖结合蛋白与弗氏不完全佐剂混合液进行皮下免疫小鼠,第9周起每周取尿测定尿蛋白,红细胞.21周处死后肾组织进行光镜、免疫荧光、电镜检测.②取动物肾组织石蜡切片进行MIF和COX-2的免疫组织化学检测.结果 ①模型鼠的尿和肾组织的检测结果与人IgAN极为相近,证实了本模型具有一定的可重复性.②MIF和COX-2在模型组中的表达显著高于其它对照组(-x分别为67和66,P均＜0.01),两者表达呈显著正相关(r=0.643,P＜0.01).结论 ①金葡菌20肽诱导的此模型,具有可重复性,并且简单、方便,可作为人IgAN相关研究的基础平台.②IgA肾病模型中MIF表达和COX-2表达增高,两者表达呈正相关,提示IgA肾病中MIF和COX-2之间有协同作用,MIF作为炎症因子可能诱导COX-2的表达.
목적 용함유금포균세포막항원결정족20태적맥아당결합단백면역Balb/c소서,중복병개진본연구소조전인이성공건립적IgAN동물모형.검측분석모형동물적빙동신조직절편상적염증인자거서세포이동억제인자(MIF)화배양화매2(COX-2)적표체급기관찰기상관성.방법 ①격주용함유20태적맥아당결합단백여불씨불완전좌제혼합액진행피하면역소서,제9주기매주취뇨측정뇨단백,홍세포.21주처사후신조직진행광경、면역형광、전경검측.②취동물신조직석사절편진행MIF화COX-2적면역조직화학검측.결과 ①모형서적뇨화신조직적검측결과여인IgAN겁위상근,증실료본모형구유일정적가중복성.②MIF화COX-2재모형조중적표체현저고우기타대조조(-x분별위67화66,P균＜0.01),량자표체정현저정상관(r=0.643,P＜0.01).결론 ①금포균20태유도적차모형,구유가중복성,병차간단、방편,가작위인IgAN상관연구적기출평태.②IgA신병모형중MIF표체화COX-2표체증고,량자표체정정상관,제시IgA신병중MIF화COX-2지간유협동작용,MIF작위염증인자가능유도COX-2적표체.
[Abstract] Objective Our study induced immunoglobulin A nephropathy (IgAN) in Balb/c mice by immunization with 20-peptide fusion protein that 20 peptide was derived from staphylococcus aureus (S aureus). Our study detected and analyzed the expression of MIF and COX-2 in frozen slice in experimental animal models. Methods (①Balb/c mice were immunized subcutaneouly with 20- peptide-fusion protein (3mg/kg) and Freunds Incomplete Adjuvant. Control groups included blank group, irrelevant-20-peptide and elute buffer immunized group. Mice were sacrificed at the end of 21 weeks and sections of the major organs were processed. Histological examination were done by light microscopy and immunofluorescence and electron microscopy detection.②MIF and COX-2 were detected with immonohistochemical method in paraffin slice of IgAN animal model. Results ①The urinalysis and nephridial tissue histopathological features in IgAN animal models were extremely similar to human IgAN. IgAN animal models were successfully established. ②The expression of MIF and COX-2 in IgAN model were remarkably higher than the other control groups(-x =67,66,P<0.01). Both of the expression had positive correlation( r = 0. 643 ,p <0.01) Conclusion ①This animal model could be regarded as the basic research platform correlated to human IgAN for its reproducibility, simple and convenient. ② The expression of MIF and COX-2 had synergistic effect as both expressions increased and had positive correlation in the IgAN animal model. MIF as inflammatory factor maybe induce the expression of COX-2.