CAJ | 학술논문

目的探讨细胞块切片免疫组织化学染色在肺癌胸腔积液检测上的临床应用价值.方法收集滨州市人民医院2009年至2011年治疗的167例胸腔积液患者,包括103例肺癌和64例肺良性疾病,对胸腔积液进行离心,制作细胞块切片HE染色及免疫组织化学染色.结果 HE染色肺癌胸腔积液和肺良性疾病胸腔积液中诊断困难比例分刺为20.4％和20.3％.GULT-1、CEA、MOC-31、Ber-EP4、TTF-1、HBME-1、CK5/6、WT-1在肺癌胸腔积液中的阳性率分别为81.3％、83.5％、87.9％、91.2％、86.8％、36.3％、0、0,在肺良性疾病胸腔积液中的阳性率分别为23.4％、0、1.5％、3.1％、0、87.5％、81.3％、84.4％.GULT-1、MOC-31及Ber-EP4在良、恶性胸腔积液中的阳性表达率比较差异有统计学意义.CK7在肺腺癌和肺良性疾病胸腔积液中阳性率分别为95.7％和96.9％,但在肺鳞癌中无一表达.结论细胞块免疫组织化学染色是诊断肺癌胸腔积液的有效方法,结合HE染色及多种免疫组织化学抗体联合使用可以显著提高诊断的阳性率.
목적 탐토세포괴절편면역조직화학염색재폐암흉강적액검측상적림상응용개치.방법 수집빈주시인민의원2009년지2011년치료적167례흉강적액환자,포괄103례폐암화64례폐량성질병,대흉강적액진행리심,제작세포괴절편HE염색급면역조직화학염색.결과 HE염색폐암흉강적액화폐량성질병흉강적액중진단곤난비례분자위20.4％화20.3％.GULT-1、CEA、MOC-31、Ber-EP4、TTF-1、HBME-1、CK5/6、WT-1재폐암흉강적액중적양성솔분별위81.3％、83.5％、87.9％、91.2％、86.8％、36.3％、0、0,재폐량성질병흉강적액중적양성솔분별위23.4％、0、1.5％、3.1％、0、87.5％、81.3％、84.4％.GULT-1、MOC-31급Ber-EP4재량、악성흉강적액중적양성표체솔비교차이유통계학의의.CK7재폐선암화폐량성질병흉강적액중양성솔분별위95.7％화96.9％,단재폐린암중무일표체.결론 세포괴면역조직화학염색시진단폐암흉강적액적유효방법,결합HE염색급다충면역조직화학항체연합사용가이현저제고진단적양성솔.
Objective To study the clinical values of immunohistochemistry staining by cell block sections in pleural effusion of patients with lung cancer.Methods One hundred and sixty-seven pleural effusion specimens were collected from Binzhou People' s Hospital.The cytospin preparations,cell block sections,HE sections and immunohistochemical study were perforated.Results The proportions of the HE sections which were difficult to make diagnosis for lung cancer and benign disease effusion were 20.4％ and 20.3％.The positive rates of GULT-1,CEA,MOC-31,Ber-EP4,TFF-1,HBME-1,CK.5/6,WT-1 in lung cancer effusion were 81.3％,83.5％,87.9％,91.2％,86.8％,36.3％,0,0,and 23.4％,0,1.5％,3.1％,O,87.5％,81.3％,84.4％ in benign disease effusion.The difference of GULT-I,MOC-31 and Ber-EP4 expressions between benign disease' s and lung cancer' s effusion was statistically significant.The positive rates of CK7 were 95.7％ and 96.9％ in lung adenocarcinoma and benign disease,but no expression in lung squamous cell carcinoma.Conclusions Immunohistochemistry,when applied on cell block sections,is useful for the diagnosis of lung cancer effusion.Combination of HE sections,and a panel of immunohistochemical markers can further improve the rate of positive diagnosis.