CAJ | 학술논문

外源一氧化氮(nitric oxide,NO)供体硝普钠(sodium nitroprusside,SNP)和过氧化氢(hydrogen peroxide,H2O2)处理抑制小麦 (Triticum aestivum L.)叶片顺乌头酸酶活性,抑制呈明显的浓度及时间效应;同时外源NO衍生代谢物过氧亚硝酸阴离子(peroxynitrite,ONOO- )的供体3-morpholinosydnonimine hydrochloride (SIN-1)和水杨酸(salicylic acid,SA)对酶活性也具有抑制作用,而且小麦叶片线粒体顺乌头酸酶对H2O2和SIN-1更敏感.分别以SNP与过氧化氢酶(catalase,CAT)专一性抑制剂氨基三唑(3-amino-1,2,4-triazole,3-AT)处理离体小麦叶片,发现在其内源H2O2含量上升的同时,顺乌头酸酶活性均呈浓度与时间依赖性下降趋势,表明NO除直接抑制顺乌头酸酶活性外,还可能经H2O2介导间接对顺乌头酸酶产生抑制作用.
외원일양화담(nitric oxide,NO)공체초보납(sodium nitroprusside,SNP)화과양화경(hydrogen peroxide,H2O2)처리억제소맥 (Triticum aestivum L.)협편순오두산매활성,억제정명현적농도급시간효응;동시외원NO연생대사물과양아초산음리자(peroxynitrite,ONOO- )적공체3-morpholinosydnonimine hydrochloride (SIN-1)화수양산(salicylic acid,SA)대매활성야구유억제작용,이차소맥협편선립체순오두산매대H2O2화SIN-1경민감.분별이SNP여과양화경매(catalase,CAT)전일성억제제안기삼서(3-amino-1,2,4-triazole,3-AT)처리리체소맥협편,발현재기내원H2O2함량상승적동시,순오두산매활성균정농도여시간의뢰성하강추세,표명NO제직접억제순오두산매활성외,환가능경H2O2개도간접대순오두산매산생억제작용.