CAJ | 학술논문

目的应用感染曼氏血吸虫(利比里亚株)的小鼠观察蒿甲醚单剂量与效应的关系,虫体肝移及蒿甲醚所引起的虫的形态学和组织病理学变化.方法感染21 d童虫的小鼠一次口服蒿甲醚12.5 mg/kg至600 mg/kg不同剂量,治后28 d剖检观察各组虫数.感染46 d或70 d成虫的小鼠一次口服蒿甲醚400 mg/kg后8～14 d,观察虫体肝移及其形态和组织病理学变化.结果蒿甲醚对21 d童虫的最低有效剂量为200 mg/kg,减虫率为81%.用蒿甲醚治疗后8 h成虫开始肝移,3～7 d全部肝移,14 d有31%的虫返回肠系膜静脉.成虫虫体萎缩,咽部扩大,肠管膨胀及其色素减少.雌虫局部体表受损,白细胞附着,卵巢及卵黄腺变性退化,以及雄虫睾丸萎缩等.在肝内的虫体被嗜酸粒细胞为主的炎细胞包围和浸润.结论蒿甲醚对小鼠曼氏血吸虫21 d童虫的最低有效剂量为200 mg/kg,可引起曼氏血吸虫成虫萎缩、退化或死亡.在肝内受损的虫体主要是被嗜酸粒细胞包围和侵袭所致.
목적응용감염만씨혈흡충(리비리아주)적소서관찰호갑미단제량여효응적관계,충체간이급호갑미소인기적충적형태학화조직병이학변화.방법감염21 d동충적소서일차구복호갑미12.5 mg/kg지600 mg/kg불동제량,치후28 d부검관찰각조충수.감염46 d혹70 d성충적소서일차구복호갑미400 mg/kg후8～14 d,관찰충체간이급기형태화조직병이학변화.결과호갑미대21 d동충적최저유효제량위200 mg/kg,감충솔위81%.용호갑미치료후8 h성충개시간이,3～7 d전부간이,14 d유31%적충반회장계막정맥.성충충체위축,인부확대,장관팽창급기색소감소.자충국부체표수손,백세포부착,란소급란황선변성퇴화,이급웅충고환위축등.재간내적충체피기산립세포위주적염세포포위화침윤.결론호갑미대소서만씨혈흡충21 d동충적최저유효제량위200 mg/kg,가인기만씨혈흡충성충위축、퇴화혹사망.재간내수손적충체주요시피기산립세포포위화침습소치.
Objective To investigate the effects of artemether on Schistosoma mansoni harboured in mice, with particular consideration on single dose-efficacy relationship, hepatic shift and artemether-induced alterations in worm morphology and histopathology. Methods Groups of mice, infected with 21 d old S. mansoni, were treated with artemether at single oral doses of 12.5 mg/kg to 600 mg/kg. Worm burden reduction was assessed 28 d post-treatment. The hepatic shift was investigated in mice infected with 46 d old S. mansoni and treated with artemether at a single oral dose of 400 mg/kg within a period of 14 d post-treatment. Morphological and histopathological observations were made in adult worms in mice, subject to single oral dose of artemether at 400 mg/kg. Results The minimum effective dose of oral artemether against juvenile worms in mice was 200 mg/kg, resulting in a worm burden reduction of 81%. The hepatic shift commenced 8 h post-treatment, and all worms shifted to the liver 3 -7 d post-treatment. Fourteen days post-treatment, 31% of the worms returned to the mesenteric veins. Treatment with artemether resulted in decreased worm body size, expansion of the pharynx and dilation of the gut with marked reduction in pigment. Focal tegumental damage was observed among female worms with adherence of host leukocytes and degeneration of ovary and vitelline glands, as well as atrophy of testis in male worms. Artemether-damaged worms were surrounded and infiltrated by eosinophils. Conclusion The minimum effective dosage of artemether against 21 d old S. mansoni in mice is 200 mg/kg. Artemether also exhibits effect against adult schistosomes, including shrinkage and degeneration, and can lead to worm death. The predominant inflammatory cell surrounded and infiltrated into the artemether-damaged worm is eosinophil.