北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2010年
1期
54-56,后插4
,共4页
王荣国%周卫%章永东%孔焕宇%李莉%林新晓%张万强
王榮國%週衛%章永東%孔煥宇%李莉%林新曉%張萬彊
왕영국%주위%장영동%공환우%리리%림신효%장만강
三七凝胶%粘连%椎板切除术%COX-2%TGF-β_1%大鼠
三七凝膠%粘連%椎闆切除術%COX-2%TGF-β_1%大鼠
삼칠응효%점련%추판절제술%COX-2%TGF-β_1%대서
Sanqi Gel%adhesion%laminectomy%cyclooxygenase-2%transforming growth factor-β_1%rats
目的 探讨三七凝胶预防硬膜外粘连(Epidural adhesion)的相关机制.方法 将72只雄性SD大鼠随机分为生理盐水组、粘连平组、凝胶组和三七凝胶组,每组18只,建立椎板切除术模型,分别在术后第7、14、21天采用免疫组化进行动态观察,分析愈合过程中TGF-β1和COX-2的表达情况.结果 不同处理方法对TGF-β_1和COX-2的影响作用不同,作用强度存在差异.其中术后14 d生理盐水组、凝胶组和粘连平组COX-2的表达显著高于三七凝胶组(P<0.05);术后7、14、21 d 3个时间点中生理盐水组、凝胶组和粘连平组TGF-β_1的表达均显著高于三七凝胶组(P<0.05).结论 三七凝胶可能通过抑制COX-2和TGF-β_1的表达预防硬膜外瘢痕粘连.
目的 探討三七凝膠預防硬膜外粘連(Epidural adhesion)的相關機製.方法 將72隻雄性SD大鼠隨機分為生理鹽水組、粘連平組、凝膠組和三七凝膠組,每組18隻,建立椎闆切除術模型,分彆在術後第7、14、21天採用免疫組化進行動態觀察,分析愈閤過程中TGF-β1和COX-2的錶達情況.結果 不同處理方法對TGF-β_1和COX-2的影響作用不同,作用彊度存在差異.其中術後14 d生理鹽水組、凝膠組和粘連平組COX-2的錶達顯著高于三七凝膠組(P<0.05);術後7、14、21 d 3箇時間點中生理鹽水組、凝膠組和粘連平組TGF-β_1的錶達均顯著高于三七凝膠組(P<0.05).結論 三七凝膠可能通過抑製COX-2和TGF-β_1的錶達預防硬膜外瘢痕粘連.
목적 탐토삼칠응효예방경막외점련(Epidural adhesion)적상관궤제.방법 장72지웅성SD대서수궤분위생리염수조、점련평조、응효조화삼칠응효조,매조18지,건립추판절제술모형,분별재술후제7、14、21천채용면역조화진행동태관찰,분석유합과정중TGF-β1화COX-2적표체정황.결과 불동처리방법대TGF-β_1화COX-2적영향작용불동,작용강도존재차이.기중술후14 d생리염수조、응효조화점련평조COX-2적표체현저고우삼칠응효조(P<0.05);술후7、14、21 d 3개시간점중생리염수조、응효조화점련평조TGF-β_1적표체균현저고우삼칠응효조(P<0.05).결론 삼칠응효가능통과억제COX-2화TGF-β_1적표체예방경막외반흔점련.
Objective To discuss the relevant mechanism of Sanqi Gel in the prevention of epidural adhesion. Method All male SD rats (n=72) were randomly divided into the normal saline water group, Zhanlianping group, carbopol gel group and Sanqi Gel group (each n=18). The model of laminectomy was established and observed at different time points after the laminectomy for 7, 14 and 21 days respectively by applying immunohistochemistry method. The expressions of transforming growth factor-β_1 (TGF-β_1) and cyclooxygenase-2 (COX-2) were analysed during the healing period.Result The different treating methods had different effects on the expressions of TGF-β_1 and COX-2 with different effective strength. The expression of COX-2 was higher significantly in the normal saline water group, carbopol gel group and Zhanlianping group than that in the Sanqi Gel group (P<0.05) 14 days after the operation. The expression of TGF-β_1 was higher significantly in the normal saline water group, carbopol gel group and Zhanlianping group than that in the Sanqi Gel group (P<0.05) at 3 time points (7, 14 and 21 days after the operation).Conclusion Sanqi Gel can prevent epidural adhesion through inhibiting the expressions of COX-2 and TGF-β_1.
