CAJ | 학술논문

目的: 体外研究不同浓度的富血小板血浆(platelet-rich plasma, PRP)对牙周膜成纤维细胞(periodontal ligament fibroblasts,PDLFs)的增殖、迁移和分化的影响.方法:不同浓度PRP(10, 50, 100, 200, 300, 500 ml/L),加入到原代培养的人PDLFs,培养时间为3、 5、 7 d,MTT法测定细胞增殖效果,transwell系统测定细胞迁移效果,AKP试剂盒测定碱性磷酸酶活性.结果:PRP有明显促进增殖作用,以200 ml/L浓度PRP效果最好,更高浓度促进作用反而减低.细胞迁移效果中,各浓度组均比阴性对照组效果好,尤其是100 ml/L的效果最佳.对于碱性磷酸酶活性,也具有明显的促进作用,以300 ml/L的效果最佳.结论:PRP在一定浓度范围内对人PDLFs功能有明显促进效果,但是在更高浓度下,这种促进效果反而减低.
목적: 체외연구불동농도적부혈소판혈장(platelet-rich plasma, PRP)대아주막성섬유세포(periodontal ligament fibroblasts,PDLFs)적증식、천이화분화적영향.방법:불동농도PRP(10, 50, 100, 200, 300, 500 ml/L),가입도원대배양적인PDLFs,배양시간위3、 5、 7 d,MTT법측정세포증식효과,transwell계통측정세포천이효과,AKP시제합측정감성린산매활성.결과:PRP유명현촉진증식작용,이200 ml/L농도PRP효과최호,경고농도촉진작용반이감저.세포천이효과중,각농도조균비음성대조조효과호,우기시100 ml/L적효과최가.대우감성린산매활성,야구유명현적촉진작용,이300 ml/L적효과최가.결론:PRP재일정농도범위내대인PDLFs공능유명현촉진효과,단시재경고농도하,저충촉진효과반이감저.
Objective:To investigate the in vitro effects of platelet-rich plasma(PRP) on human periodontal ligament fibroblasts(PDLFs). Methods: Various concentrations of PRP (10, 50, 100, 200, 300, 500 ml/L) were applied to primary cultures of human PDLFs. MTT assays were utilized to assess cell proliferation ability. Migration was determined by assessing the cell response to a concentration gradient with Transwell chamber. Differentiation was assessed using alkaline phosphatase (ALP) kit. Results: A beneficial effect on proliferation was observed, especially in response to 200 ml/L PRP.PRP had stimulatory effects on the migration of human PDLFs. PRP facilitated differentiation of PDLFs. Conclusion: PRP can exert a positive effect on human PDLFs,but this effect is concentration specific, while higher concentrations is not necessary to result in optimal outcomes.