CAJ | 학술논문

目的:研究Survivin、Bcl-2、HPV 16/18在宫颈上皮内瘤变(CIN)及宫颈癌中的表达,探讨三者与宫颈癌的相关性.方法:采用原位杂交法检测正常宫颈组织(对照组)、CIN(CIN组)和宫颈癌(宫颈癌组)中Survivin mRNA及HPV 16/18 DNA的表达.采用免疫组织化学法检测Bcl-2蛋白在各组中的表达.结果:①Bcl-2蛋白、HPV16/18DNA、Survivin mRNA的阳性率在对照组、CIN组、宫颈癌组中逐渐升高,差异有高度统计学意义(P=0.000).②Bcl-2蛋白和Survivin mRNA在宫颈癌的组织分化程度中,低分化组的表达高于高、中分化组(P<0.01),ⅡB～Ⅲ期显著高于I～ⅡA期(P<0.05);Survivin mRNA在淋巴结转移组中高于无淋巴结转移组(P<0.01);Survivin mRNA及Bcl-2与组织类型及肿块类型无关(P>0.05);HPV 16/18感染与宫颈癌组织类型、组织分化程度、临床分期、肿块类型均无关(P>0.05).(③Survivin与Bcl-2及HPV 16/18在宫颈癌中的表达均呈正相关.结论:Survivin、Bcl-2及HPV 16/18在宫颈癌中有异常表达.三者可能与宫颈癌的发生发展有密切关系.
목적:연구Survivin、Bcl-2、HPV 16/18재궁경상피내류변(CIN)급궁경암중적표체,탐토삼자여궁경암적상관성.방법:채용원위잡교법검측정상궁경조직(대조조)、CIN(CIN조)화궁경암(궁경암조)중Survivin mRNA급HPV 16/18 DNA적표체.채용면역조직화학법검측Bcl-2단백재각조중적표체.결과:①Bcl-2단백、HPV16/18DNA、Survivin mRNA적양성솔재대조조、CIN조、궁경암조중축점승고,차이유고도통계학의의(P=0.000).②Bcl-2단백화Survivin mRNA재궁경암적조직분화정도중,저분화조적표체고우고、중분화조(P<0.01),ⅡB～Ⅲ기현저고우I～ⅡA기(P<0.05);Survivin mRNA재림파결전이조중고우무림파결전이조(P<0.01);Survivin mRNA급Bcl-2여조직류형급종괴류형무관(P>0.05);HPV 16/18감염여궁경암조직류형、조직분화정도、림상분기、종괴류형균무관(P>0.05).(③Survivin여Bcl-2급HPV 16/18재궁경암중적표체균정정상관.결론:Survivin、Bcl-2급HPV 16/18재궁경암중유이상표체.삼자가능여궁경암적발생발전유밀절관계.
Objective:The expression of survivin、Bcl-2 and HPV16/18 in cervical intraepithelial neoplasia and cervical cancer were investigated,and the correlation of these factors with cervical cancer was explored.Methods.The expression of survivin mRNA and HPV16/18 DNA in normal cervical tissues,CIN and cervical cancer was detected by hybridization in situ.The expression of Bcl-2 was analyzed by immunohistochemistry.Results:The positive rate of survivin mRNA、Bcl-2 protein and HPV16/18 DNA were raised gradually from normal cervical tissues to CIN and to Cervical cancer.Survivin mRNA and Bcl-2 protein had a significant higher expression in poor differentiation and higher stages(ⅡB～Ⅲ),while the pathological and histologic types had no difference.The expression of survivin mRNA in Cervical cancer with lymphatic metastasis was much higher than that without lymphatic metastasis.There was no correlation of HPV16/18 infection with clinical stages,pathological types,histological types or differentiation of cervical cancer.Conclusions:The expressions of Survivin mRNA、Bcl-2 and HPV16/18DNA were abnormal in Cervical cancer which might be the clue of development of cervical cancer.