CAJ | 학술논문

利用热激PCR法和菌落直接PCR法进行大肠杆菌和农杆菌的鉴定,筛选含有GFP-linker-C-TAPa或wtGPA1-C-TAPa的pCAMBIA2300EC的阳性克隆,比较热激PCR法和菌落直接PCR法的优劣.热激PCR法阳性检出率是100%,菌落直接PCR法阳性检出率是60%左右.热激PCR法是一种快速可靠的鉴定重组阳性克隆的方法.
이용열격PCR법화균락직접PCR법진행대장간균화농간균적감정,사선함유GFP-linker-C-TAPa혹wtGPA1-C-TAPa적pCAMBIA2300EC적양성극륭,비교열격PCR법화균락직접PCR법적우렬.열격PCR법양성검출솔시100%,균락직접PCR법양성검출솔시60%좌우.열격PCR법시일충쾌속가고적감정중조양성극륭적방법.
To compare advantage and disadvantage of microbe screen between heat shock PCR method and colony direct PCR method,the positive clones of pCAMBIA2300EC with GFP-linker-C-TAPa or wtGPAI-C-TAPa were screened by heat shock PCR method and colony direct PCR method in Escherichia coil and Agrobacterium tumefaciens respectively.The positive rates of screening clones by heat shock PCR method were 100%.The positive rates of screening clones by colony direct PCR method were about 60%.Result showed that heat shock PCR method is effective way for microbe screen.