国际医药卫生导报
國際醫藥衛生導報
국제의약위생도보
INTERNATIONAL MEDICINE & HEALTH GUIDANCE NEWS
2012年
7期
889-892
,共4页
陆喜颜%刘美真%陈经雕%李柏生%柯碧霞%谭海玲%张万里%梁剑
陸喜顏%劉美真%陳經彫%李柏生%柯碧霞%譚海玲%張萬裏%樑劍
륙희안%류미진%진경조%리백생%가벽하%담해령%장만리%량검
PCR%流行性脑脊髓膜炎%奈瑟球菌%鉴定
PCR%流行性腦脊髓膜炎%奈瑟毬菌%鑒定
PCR%류행성뇌척수막염%내슬구균%감정
PCR%Epidemic cerebrospinal meningitis%Neisseria meningitidis%Identification
目的 用PCR技术鉴定分析广东省2003-2011年从流脑患者、密切接触者及健康带菌者分离的C群流脑菌株.方法 对菌株进行常规鉴定、乳胶凝集,再用PCR方法进行鉴定.结果 36株脑膜炎奈瑟菌常规鉴定、乳胶凝集为C群,PCR鉴定crgA基因(230 bp)、siaD(C)基因( 250 bp)均为阳性.结论 基于siaD(C)基因的PCR技术可应用于C群脑膜炎奈瑟菌的鉴定分群.
目的 用PCR技術鑒定分析廣東省2003-2011年從流腦患者、密切接觸者及健康帶菌者分離的C群流腦菌株.方法 對菌株進行常規鑒定、乳膠凝集,再用PCR方法進行鑒定.結果 36株腦膜炎奈瑟菌常規鑒定、乳膠凝集為C群,PCR鑒定crgA基因(230 bp)、siaD(C)基因( 250 bp)均為暘性.結論 基于siaD(C)基因的PCR技術可應用于C群腦膜炎奈瑟菌的鑒定分群.
목적 용PCR기술감정분석광동성2003-2011년종류뇌환자、밀절접촉자급건강대균자분리적C군류뇌균주.방법 대균주진행상규감정、유효응집,재용PCR방법진행감정.결과 36주뇌막염내슬균상규감정、유효응집위C군,PCR감정crgA기인(230 bp)、siaD(C)기인( 250 bp)균위양성.결론 기우siaD(C)기인적PCR기술가응용우C군뇌막염내슬균적감정분군.
Objective To identify and analyze the strains of Neisseria meningitidis serogroup C isolated from patients with of Neisseria meningitidis,close contact individuals,and healthy carriers during the period of 2003 to 2011 in Guangdong province by PCR technology.Methods The strains were identified by routine methods,then latex aggregation,and finally by polymerase chain reaction ( PCR ).Results 36 strains were identified as serogroup C via conventional methods and latex agglutinate crgA gene ( 230 bp ) and siaD ( C ) gene ( 250 bp ) were all postive on PCR identication.Conclusions siaD( C ) gene-based PCR technology can be applied in identification of Neisseria meningitidis serogroup C.
