肿瘤研究与临床
腫瘤研究與臨床
종류연구여림상
CANCER RESEARCH AND CLINIC
2010年
5期
300-302
,共3页
吴相稳%李辉%胡滨%苗劲柏%贾兴元
吳相穩%李輝%鬍濱%苗勁柏%賈興元
오상은%리휘%호빈%묘경백%가흥원
半乳糖凝集素3%骨桥蛋白质%食管肿瘤%微转移
半乳糖凝集素3%骨橋蛋白質%食管腫瘤%微轉移
반유당응집소3%골교단백질%식관종류%미전이
Galectin-3%Osteopontin%Esophageal neoplasms%Micrometastasis
目的 研究半乳糖凝集素-3(Gal-3)、骨桥蛋白(OPN)在食管癌中的表达情况及其临床意义.方法 选取2006年至2008年接受手术治疗的食管癌患者37例,男28例,女9例,年龄41～82岁.应用荧光PCR及ELISA方法分别检测食管癌组织、癌旁组织及正常食管组织Gal-3 mRNA、OPN mRNA和血浆中Gal-3、OPN的水平,并结合术后随访,对其表达差异进行分析.结果 37例食管癌患者的肿瘤组织与癌旁组织及正常食管组织中Gal-3 mRNA相对表达值差异有统计学意义(F=11.934,P<0.05).三者之间OPN mRNA相对表达值差异有统计学意义(F=4.269,P<0.05).不同分期患者的食管癌组织Gal-3mRNA和OPN mRNA表达差异有统计学意义(F=3.216,P<0.05),而与患者的年龄、性别和肿瘤病理性质无关.复发组血浆中Gal-3和OPN水平及癌组织中的Gal-3 mRNA和OPN mRNA表达均高于未复发组(P<O.05).结论 食管癌组织中(Gal-3 mRNA和OPN mRNA的差异表达可能与食管癌的发生、发展和转移有关.术前外周血Gal-3和OPN检测可能成为判定食管癌术前微转移的重要指标之一.
目的 研究半乳糖凝集素-3(Gal-3)、骨橋蛋白(OPN)在食管癌中的錶達情況及其臨床意義.方法 選取2006年至2008年接受手術治療的食管癌患者37例,男28例,女9例,年齡41～82歲.應用熒光PCR及ELISA方法分彆檢測食管癌組織、癌徬組織及正常食管組織Gal-3 mRNA、OPN mRNA和血漿中Gal-3、OPN的水平,併結閤術後隨訪,對其錶達差異進行分析.結果 37例食管癌患者的腫瘤組織與癌徬組織及正常食管組織中Gal-3 mRNA相對錶達值差異有統計學意義(F=11.934,P<0.05).三者之間OPN mRNA相對錶達值差異有統計學意義(F=4.269,P<0.05).不同分期患者的食管癌組織Gal-3mRNA和OPN mRNA錶達差異有統計學意義(F=3.216,P<0.05),而與患者的年齡、性彆和腫瘤病理性質無關.複髮組血漿中Gal-3和OPN水平及癌組織中的Gal-3 mRNA和OPN mRNA錶達均高于未複髮組(P<O.05).結論 食管癌組織中(Gal-3 mRNA和OPN mRNA的差異錶達可能與食管癌的髮生、髮展和轉移有關.術前外週血Gal-3和OPN檢測可能成為判定食管癌術前微轉移的重要指標之一.
목적 연구반유당응집소-3(Gal-3)、골교단백(OPN)재식관암중적표체정황급기림상의의.방법 선취2006년지2008년접수수술치료적식관암환자37례,남28례,녀9례,년령41～82세.응용형광PCR급ELISA방법분별검측식관암조직、암방조직급정상식관조직Gal-3 mRNA、OPN mRNA화혈장중Gal-3、OPN적수평,병결합술후수방,대기표체차이진행분석.결과 37례식관암환자적종류조직여암방조직급정상식관조직중Gal-3 mRNA상대표체치차이유통계학의의(F=11.934,P<0.05).삼자지간OPN mRNA상대표체치차이유통계학의의(F=4.269,P<0.05).불동분기환자적식관암조직Gal-3mRNA화OPN mRNA표체차이유통계학의의(F=3.216,P<0.05),이여환자적년령、성별화종류병이성질무관.복발조혈장중Gal-3화OPN수평급암조직중적Gal-3 mRNA화OPN mRNA표체균고우미복발조(P<O.05).결론 식관암조직중(Gal-3 mRNA화OPN mRNA적차이표체가능여식관암적발생、발전화전이유관.술전외주혈Gal-3화OPN검측가능성위판정식관암술전미전이적중요지표지일.
Objective To investigate the galectin-3 (Gal-3) and osteopontin (OPN) expression in esophageal carcinoma and analyze its clinical significance.Methods Thirty-seven patients who suffered from esophageal carcinoma were selected from 2006 to 2008.There were 28 males and 9 females,aged from 41 to 82 vears old.The levels of Gal-3 mRNA and OPN mRNA in tissues were detected by Fluorescent PCR,and the levels of Gal-3 and OPN in blood plasma were measured by ELISA.Result The expression of Gal-3 mRNA and OPN mRNA were significantly different between the esophageal carcinoma,side of carcinoma and normal esophagus tissues (F=11.934,F=4.269,P<0.05);The expression of galectin-3 and osteopontin were correlated with pathological grading (F=3.216,P<O.05),but not with the patient's age,gender and pathologic type.In metastasis group,the Gal-3 mRNA and OPN mRNA expression levels were significantly higher that that in un-metastasis group (P<O.05).Conclusion Different expression of Gal-3 and OPN may be related to the incidence.development and metastasis of esophageal cancer.Detection of Gal-3 and OPN of preoperative peripheral blood could be one of the important indicators,which could determine micrometastasis of esophageal carcinoma before operation.
