CAJ | 학술논문

[目的]检测鸡胚不同发育阶段时的DNA甲基化水平.[方法]以不同发育阶段的家鸡胚胎为试验材料,提取基因组DNA,采用甲基敏感扩增片段多态性(methylation sensitive amplified polymorphism,MSAP)技术对其甲基化水平进行了初步检测.[结果]共发现甲基化位点880个, CCGG位点单链外侧的胞嘧啶甲基化位点比例为20.48%,双链CCGG位点的内侧胞嘧啶甲基化19.30%,两者总比例为39.78%.[结论]鸡胚发育过程,DNA甲基化随着胚胎的发育呈升高趋势.
[목적]검측계배불동발육계단시적DNA갑기화수평.[방법]이불동발육계단적가계배태위시험재료,제취기인조DNA,채용갑기민감확증편단다태성(methylation sensitive amplified polymorphism,MSAP)기술대기갑기화수평진행료초보검측.[결과]공발현갑기화위점880개, CCGG위점단련외측적포밀정갑기화위점비례위20.48%,쌍련CCGG위점적내측포밀정갑기화19.30%,량자총비례위39.78%.[결론]계배발육과정,DNA갑기화수착배태적발육정승고추세.
[Objective]The study aimed to detect the DNA methylation level in different chicken fetation stages. [Method]The chicken embryo in different development stages was taken as the tested materials to extract the genomic DNA and its DNA methylation level was detected preliminarily by the technique of methylation sensitive amplified polymorphism (MSAP). [Result]It was found that the number of methylation sites were 880, in which, the single-stranded outboard cytosine methylation (hmCCGG) sites of CCGG sites accounted for 20.48%, the double-stranded inside cytosine methylation (Cm CGG) sites of CCGG sites accounted for 19.30%, resp., and the total of them was 39.78%. [Conclusion]The level of DNA methylation showed a rising trend along with the chicken fetation.