临床荟萃
臨床薈萃
림상회췌
CLINICAL FOCUS
2009年
16期
1388-1391
,共4页
李雪峰%秦贵军%李志臻%饶小娟%马晓君
李雪峰%秦貴軍%李誌臻%饒小娟%馬曉君
리설봉%진귀군%리지진%요소연%마효군
肥胖症%转录因子%葡萄糖转运体4型%胰岛素抗体
肥胖癥%轉錄因子%葡萄糖轉運體4型%胰島素抗體
비반증%전록인자%포도당전운체4형%이도소항체
obesity%transcription factors%glucose transporter type 4%insulin antibodies
目的 观察肥胖者网膜脂肪组织中叉头状转录因子O1(FOXO1)、葡萄糖转运体4(GLUT4)mRNA表达,探讨FOXO1在肥胖和胰岛素抵抗发生中的作用.方法 聚集15例肥胖者和17例非肥胖者的网膜脂肪组织应用半定量反转录聚合酶链反应(RT-PCR)测定FOXO1、GLUT4 mRNA表达,并测定其他临床指标,分析各指标之间的相关性及与胰岛素敏感性的关系.结果 肥胖者FOXO1mRNA的表达显著高于非肥胖对照组,(0.577±0.038 vs0.359±0.023)(P<0.01),GLUT4mRNA的表达明显低于非肥胖对照组,(0.386±0.037 vs 0.646±0.034)(P<0.01);网膜脂肪组织FOXO1 mRNA的表达与体质量指数(BMI)、腰臀比(WHR)、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、甘油三酯(TG)的表达呈正相关(r=0.963、0.939、0.974、0.924、0.600,均P<0.01),与GLUT4mRNA的表达呈负相关(r=-0.866,P<0.01),多元逐步回归分析示BMI、HOMA-IR、GLUT4 mRNA为FOXO1mRNA的独立相关因素.结论 肥胖者的网膜脂肪组织中的FOXO1表达明显增加,FOXO1可能是肥胖和胰岛素抵抗的联系者,可能是通过减少GLUT4的表达引起肥胖者胰岛素抵抗的.
目的 觀察肥胖者網膜脂肪組織中扠頭狀轉錄因子O1(FOXO1)、葡萄糖轉運體4(GLUT4)mRNA錶達,探討FOXO1在肥胖和胰島素牴抗髮生中的作用.方法 聚集15例肥胖者和17例非肥胖者的網膜脂肪組織應用半定量反轉錄聚閤酶鏈反應(RT-PCR)測定FOXO1、GLUT4 mRNA錶達,併測定其他臨床指標,分析各指標之間的相關性及與胰島素敏感性的關繫.結果 肥胖者FOXO1mRNA的錶達顯著高于非肥胖對照組,(0.577±0.038 vs0.359±0.023)(P<0.01),GLUT4mRNA的錶達明顯低于非肥胖對照組,(0.386±0.037 vs 0.646±0.034)(P<0.01);網膜脂肪組織FOXO1 mRNA的錶達與體質量指數(BMI)、腰臀比(WHR)、空腹胰島素(FINS)、胰島素牴抗指數(HOMA-IR)、甘油三酯(TG)的錶達呈正相關(r=0.963、0.939、0.974、0.924、0.600,均P<0.01),與GLUT4mRNA的錶達呈負相關(r=-0.866,P<0.01),多元逐步迴歸分析示BMI、HOMA-IR、GLUT4 mRNA為FOXO1mRNA的獨立相關因素.結論 肥胖者的網膜脂肪組織中的FOXO1錶達明顯增加,FOXO1可能是肥胖和胰島素牴抗的聯繫者,可能是通過減少GLUT4的錶達引起肥胖者胰島素牴抗的.
목적 관찰비반자망막지방조직중차두상전록인자O1(FOXO1)、포도당전운체4(GLUT4)mRNA표체,탐토FOXO1재비반화이도소저항발생중적작용.방법 취집15례비반자화17례비비반자적망막지방조직응용반정량반전록취합매련반응(RT-PCR)측정FOXO1、GLUT4 mRNA표체,병측정기타림상지표,분석각지표지간적상관성급여이도소민감성적관계.결과 비반자FOXO1mRNA적표체현저고우비비반대조조,(0.577±0.038 vs0.359±0.023)(P<0.01),GLUT4mRNA적표체명현저우비비반대조조,(0.386±0.037 vs 0.646±0.034)(P<0.01);망막지방조직FOXO1 mRNA적표체여체질량지수(BMI)、요둔비(WHR)、공복이도소(FINS)、이도소저항지수(HOMA-IR)、감유삼지(TG)적표체정정상관(r=0.963、0.939、0.974、0.924、0.600,균P<0.01),여GLUT4mRNA적표체정부상관(r=-0.866,P<0.01),다원축보회귀분석시BMI、HOMA-IR、GLUT4 mRNA위FOXO1mRNA적독립상관인소.결론 비반자적망막지방조직중적FOXO1표체명현증가,FOXO1가능시비반화이도소저항적련계자,가능시통과감소GLUT4적표체인기비반자이도소저항적.
Objective To determine the effect of forkhead transcription factor O1 (FOXO1) on insulin resistance by detecting the expression of FOXO1 and glucose transporter 4(GLUT4) mRNA in omental adipose tissue of obese individuals. Methods The expression of FOXO1 and GLUT4 mRNA in omental adipose tissue of individuals with obesity(15 cases) and the controls(without obesity) (17 cases) were detected by using reverse transcription-polymerase chain reaction (RT-PCR) ,and other clinical parameters were determined for their relationship analysis. Results Level of FOXO1 mRNA in obese individuals was significantly higher than that of the controls, (0. 577±0.038 vs 0. 359± 0. 023)(P<0.01), level of GLUT4 mRNA in obese individuals was significantly lower than that of the controls, (0. 386±0.037 vs 0. 646±0.034)(P<0.01). Pearson correlation analysis showed that the expression of FOXO1 mRNA in omental adipose tissue was positively correlated with body mass index(BMI), waist to hip ratio(WHR), fasting serum insulin(FINS), Homeostasis Model Assessment-Insulin Resistance Index(HOMA-IR) and triglyceride (TG) (r = 0. 963,0. 939,0. 974,0. 924,0. 600, all P< 0.01), but negatively correlated with GLUT4 mRNA (r = -0. 866, P<0.01). Multiple stepwise regression analysis revealed that the independent variables associated with FoxO1 mRNA were BMI, HOMA-IR, GLUT4 mRNA. Conclusion The expression of FoxO1 gene increased in the omental adipose tissue of obese individuals. FOXO1 may play an important role in the pathophysiology of insulin resistance induced by high-fat. FoxO1 may lead to the occurrence of insulin resistance partly by decreasing the expression of GLUT4.
