CAJ | 학술논문

目的:研究磷脂酰肌醇3-激酶(PI3K)信号通路在肝癌细胞血管内皮生长因子(VEGF)表达调控中的作用.方法: 体外培养高转移性人肝癌细胞株HCCLM3,分为对照组、PI3K特异性抑制剂LY294002浓度5μmol/L组,LY294002浓度10μmol/L及20μmol/L组,处理6小时后,采用逆转录聚合酶链反应(RT-PCR)技术检测VEGF mRNA表达的变化.结果: LY294002可使肝癌细胞HCCLM3的VEGF mRNA的表达下降,且这种抑制作用随着LY294002浓度增大而增大(P<0.01),呈现剂量移赖性关系.结论: LY294002可以抑制肝癌细胞株HCCLM3中VEGF的表达,肝癌细胞VEGF的表达调控受PI3K信号通路调控.
목적:연구린지선기순3-격매(PI3K)신호통로재간암세포혈관내피생장인자(VEGF)표체조공중적작용.방법: 체외배양고전이성인간암세포주HCCLM3,분위대조조、PI3K특이성억제제LY294002농도5μmol/L조,LY294002농도10μmol/L급20μmol/L조,처리6소시후,채용역전록취합매련반응(RT-PCR)기술검측VEGF mRNA표체적변화.결과: LY294002가사간암세포HCCLM3적VEGF mRNA적표체하강,차저충억제작용수착LY294002농도증대이증대(P<0.01),정현제량이뢰성관계.결론: LY294002가이억제간암세포주HCCLM3중VEGF적표체,간암세포VEGF적표체조공수PI3K신호통로조공.
Objective:To investigate the roles of phosphatidylinositol 3-kinase(PI3K) in the regulation of expression of vascular endothelial growth factor(VEGF) in hepatocellular carcinoma(HCC) cells. Methods: The high metastasic cell line HCCLM3 were cultured in DMEM medium,HCCLM3 cells were divided into control groups and LY294002 groups(5μM,10μM,20μM),LY294002 was the specific inhibitor of PI3K.The expression of VEGF mRNA was detected by reverse transcriptional polymerase chain reaction(RT-PCR) after 6 hours. Results: In HCCLM3 cells,the expression of VEGF mRNA can be apparently inhibited by LY294002. With the increase of LY294002 concentration,the expression of VEGF mRNA decreased gradually(P<0.01)and in a dose-dependent manner. Conclusion: LY294002 can reduce the expression of VEGF mRNA in HCCLM3 cell line,the expression of VEGF in HCC cells is regulated by PI3K.