CAJ | 학술논문

本文运用ARDRA(amplified ribosomal DNA restriction analysis)技术研究了冷藏鸡肉胴体中的细菌多样性.采用改良CTAB法提取两批冷藏鸡肉胴体(冷藏60 d和90 d)的细菌宏基因组DNA来构建16S rDNA文库Ⅰ和Ⅱ,从中分别随机挑取57和71个阳性克隆子用Afa和Msp进行扩增rDNA限制性分析和UPGMA聚类分析.结果表明:从文库Ⅰ和Ⅱ中分别获得47和55个特异性条带、44和65个操作分类单元(OTUs),分别占总数的77.20%和91.50%,可见在构建的文库中细菌群落结构复杂,多样性高.文库Ⅰ和Ⅱ的细菌群落丰富度指数、香-威指数、辛普森指数、均匀度指数分别为13.36、3.64、0.97、0.91和16.19、4.15、0.98、0.98,表明文库Ⅱ的细菌多样性高于文库Ⅰ.
본문운용ARDRA(amplified ribosomal DNA restriction analysis)기술연구료랭장계육동체중적세균다양성.채용개량CTAB법제취량비랭장계육동체(랭장60 d화90 d)적세균굉기인조DNA래구건16S rDNA문고Ⅰ화Ⅱ,종중분별수궤도취57화71개양성극륭자용Afa화Msp진행확증rDNA한제성분석화UPGMA취류분석.결과표명:종문고Ⅰ화Ⅱ중분별획득47화55개특이성조대、44화65개조작분류단원(OTUs),분별점총수적77.20%화91.50%,가견재구건적문고중세균군락결구복잡,다양성고.문고Ⅰ화Ⅱ적세균군락봉부도지수、향-위지수、신보삼지수、균균도지수분별위13.36、3.64、0.97、0.91화16.19、4.15、0.98、0.98,표명문고Ⅱ적세균다양성고우문고Ⅰ.
ARDRA (amplified ribosomal DNA restriction analysis)technique was applied to investigate bacterial diversity of frozen chicken during storage.Metagenome DNA from two batches of chicken sample of 60 and 90 days storage respectively was extracted to build bacterial 16S rDNA library Ⅰ and Ⅱ,from which 57 and 71 positive clones were chosen randomly from each corresponding library to analyze amplified ribosomal DNA restriction polymorphism by double digestion of A fa and Msp followed by UPGMA cluster analysis.A total of 47 and 55 distinctive bands were given by 16S rDNA-ARDRA,while 44 and 65 OTUs,accounting 77.20% and 91.50% of total clones,were obtained from library Ⅰ andⅡ,respectively.Richness index,Shannon-Wiener index,Simpson index,and Evenness index of library Ⅰ and Ⅱ were 13.36,3.64,0.97,0.91 and 16.19,4.15,0.98,0.98,respectively,suggesting that the diversity and structures of bacteria community of chicken carcass were complex,and bacterial diversity decreased with the storage time.