CAJ | 학술논문

以现蕾期新鲜苜蓿Medicago sativa为材料,采用温度、pH值单因素处理及温度、加热时间、pH值和NaCl质量分数多因素处理,探讨不同方法对苜蓿叶蛋白凝聚效果的影响.结果表明,在单因素条件下,温度以75 ℃为宜,pH值4.0对苜蓿叶蛋白产量、叶蛋白纯度和粗蛋白提取率有显著影响.而在多因素条件下,叶蛋白的最适凝聚组合条件为温度85 ℃,时间2 min,pH值4.0,以及NaCl质量分数0.4%.
이현뢰기신선목숙Medicago sativa위재료,채용온도、pH치단인소처리급온도、가열시간、pH치화NaCl질량분수다인소처리,탐토불동방법대목숙협단백응취효과적영향.결과표명,재단인소조건하,온도이75 ℃위의,pH치4.0대목숙협단백산량、협단백순도화조단백제취솔유현저영향.이재다인소조건하,협단백적최괄응취조합조건위온도85 ℃,시간2 min,pH치4.0,이급NaCl질량분수0.4%.
This paper took fresh alfalfa at bud stage as material, discussed the effects of different measures on alfalfa leaf protein extraction including temperature, pH and the orthogonal experimental design of four-factors including temperature, heating time, pH and NaCl concentration. The results indicated that in the single factor experiment, the optimum temperature and pH, which affected LPC production, LPC purity and crude protein extraction ratio, were 75 ℃and 4.0, respectively. While in the orthogonal experiment, the optimum combination factors for extracting LPC were that pH was 4.0, temperature was 85 ℃, heating time was 2 min, and NaCl concentration was 0.4%.