重庆医科大学学报
重慶醫科大學學報
중경의과대학학보
UNIVERSITATIS SCIENTIAE MEDICINAE CHONGQING
2010年
2期
209-212
,共4页
溶血磷脂酸%环氧化酶-2%顺铂%NS398%3AO细胞%卵巢癌
溶血燐脂痠%環氧化酶-2%順鉑%NS398%3AO細胞%卵巢癌
용혈린지산%배양화매-2%순박%NS398%3AO세포%란소암
Lysophosphatidic acid%Cyclooxygenase-2%Cisplatin%NS398%3AO cells%Ovarian cancer
目的:观察环氧化酶-2(Cyclooxygenase-2,COX-2)抑制剂NS398对溶血磷脂酸(Lysophosphatidic acid,LPA)拮抗顺铂抑制卵巢癌细胞细胞增殖作用的影响,并探讨其可能机制.方法:体外培养人卵巢癌细胞3AO,MTY法检测NS398对LPA拮抗顺铂抑制3AO细胞增殖的影响;流式细胞仪检测细胞周期变化;RT-PCR检测LPA单独或合用NS398对3AO细胞表达COX-2的影响.结果:LPA对顺铂抑制卵巢癌细胞增殖有拮抗作用,并降低G_0/G_1期的细胞比率;联合用NS398后,卵巢癌细胞的生长显著受抑制,同时G_0/G_1期细胞比率上升;RT-PCR结果显示40μmol/L LPA能促进COX-2的表达,而合用NS398后COX-2表达降低.结论:NS398可能通过抑制COX-2表达,实现逆转LPA拮抗顺铂抑制卵巢癌细胞细胞增殖的作用.
目的:觀察環氧化酶-2(Cyclooxygenase-2,COX-2)抑製劑NS398對溶血燐脂痠(Lysophosphatidic acid,LPA)拮抗順鉑抑製卵巢癌細胞細胞增殖作用的影響,併探討其可能機製.方法:體外培養人卵巢癌細胞3AO,MTY法檢測NS398對LPA拮抗順鉑抑製3AO細胞增殖的影響;流式細胞儀檢測細胞週期變化;RT-PCR檢測LPA單獨或閤用NS398對3AO細胞錶達COX-2的影響.結果:LPA對順鉑抑製卵巢癌細胞增殖有拮抗作用,併降低G_0/G_1期的細胞比率;聯閤用NS398後,卵巢癌細胞的生長顯著受抑製,同時G_0/G_1期細胞比率上升;RT-PCR結果顯示40μmol/L LPA能促進COX-2的錶達,而閤用NS398後COX-2錶達降低.結論:NS398可能通過抑製COX-2錶達,實現逆轉LPA拮抗順鉑抑製卵巢癌細胞細胞增殖的作用.
목적:관찰배양화매-2(Cyclooxygenase-2,COX-2)억제제NS398대용혈린지산(Lysophosphatidic acid,LPA)길항순박억제란소암세포세포증식작용적영향,병탐토기가능궤제.방법:체외배양인란소암세포3AO,MTY법검측NS398대LPA길항순박억제3AO세포증식적영향;류식세포의검측세포주기변화;RT-PCR검측LPA단독혹합용NS398대3AO세포표체COX-2적영향.결과:LPA대순박억제란소암세포증식유길항작용,병강저G_0/G_1기적세포비솔;연합용NS398후,란소암세포적생장현저수억제,동시G_0/G_1기세포비솔상승;RT-PCR결과현시40μmol/L LPA능촉진COX-2적표체,이합용NS398후COX-2표체강저.결론:NS398가능통과억제COX-2표체,실현역전LPA길항순박억제란소암세포세포증식적작용.
Objective:To investigate the effect of NS398,cyclcoxygenase-2(COX-2)-inhibitor,on the antergy of lysophosphatidic acid (LPA) for the inhibiting effect of cisplatin(DDP)in the proliferation of huanman ovarian cancer,and to analyze its possible mechanism.Methods:Human ovarian cancer cell line(3AO) were cultured in vitro.The effect of NS398 on the antergy of LPA for the inhibiting effect of DDP in the proliferation of 3A0 cells were measured by MTT Cell cycle of 3AO were analysed by flow cytometry(FCM).The effect of LPA alone or in combinafion with NS398 on the expression of COX-2 of 3AO cells were measured with RT-PCR.Resuls:LPA showed antergy on the inhibiting effect of DDP in the proliferation of 3AO cells,and decrease the proportion of cells in the G_0/G_1 phase of the cell cycle.Whereas,combined with NS398 in culture,the proliferation of were signitlcantly inhibited,and the the proportion of cells in the G_0/G_1 phase of the cell cycle was raised.RT-PCR results revealed that 40μmol/L LPA could promote the expression of COX-2 in 3AO cells,and in combination with NS398,the expression of COX-2 was decreased.Conclusion:The reverse effect of NS398 for the antergy of LPA on the inhibiting effect of DDP in the proliferation of 3AO cells may be completed by inhibiting the expression of COX-2 in these cells.
