CAJ | 학술논문

对红色、黄色、粉紫色和白色菊花品种不同开放度的花序舌状花中CHS、CHI、DFR、F3H、F3'H和3GT基因的表达量进行了相对定量分析.结果表显示:6个基因的表达因不同花色、不同发育阶段而异.'钟山红鹰'(红色)中各基因的表达量均较高,且均在Ⅱ(松蕾期)或Ⅲ(半开期) 期达到峰值,其中DFR、3GT基因的表达量远高于其他花色品种.'金陵娇黄'(黄色)中CHS、CHI基因表达量较高,且Ⅰ(紧蕾期)、Ⅱ期表达量高于Ⅲ、Ⅳ(盛开期)期;3GT、DFR基因表达量分别高或低于'金陵笑靥'(粉紫色)品种中相应基因的表达量,但均比红色品种低;F3H在4个品种中表达量最低,F3'H表达量接近或略低于红色或粉紫色品种,且各阶段表达水平较稳定.'金陵笑靥'中DFR表达量仅次于'钟山红鹰',3GT和CHS表达量低于红色与黄色品种.'钟山雪桂'(白色)中各基因仅有微量表达,除F3H外各基因的表达量明显低于其他花色品种.研究表明,花色素结构基因DFR、3GT是菊花花色素合成的关键基因,DFR很可能是限速关键基因,一定表达水平的CHS、CHI也是菊花花色素合成所必须的,F3H基因与花色素合成不存在直接相关.
대홍색、황색、분자색화백색국화품충불동개방도적화서설상화중CHS、CHI、DFR、F3H、F3'H화3GT기인적표체량진행료상대정량분석.결과표현시:6개기인적표체인불동화색、불동발육계단이이.'종산홍응'(홍색)중각기인적표체량균교고,차균재Ⅱ(송뢰기)혹Ⅲ(반개기) 기체도봉치,기중DFR、3GT기인적표체량원고우기타화색품충.'금릉교황'(황색)중CHS、CHI기인표체량교고,차Ⅰ(긴뢰기)、Ⅱ기표체량고우Ⅲ、Ⅳ(성개기)기;3GT、DFR기인표체량분별고혹저우'금릉소엽'(분자색)품충중상응기인적표체량,단균비홍색품충저;F3H재4개품충중표체량최저,F3'H표체량접근혹략저우홍색혹분자색품충,차각계단표체수평교은정.'금릉소엽'중DFR표체량부차우'종산홍응',3GT화CHS표체량저우홍색여황색품충.'종산설계'(백색)중각기인부유미량표체,제F3H외각기인적표체량명현저우기타화색품충.연구표명,화색소결구기인DFR、3GT시국화화색소합성적관건기인,DFR흔가능시한속관건기인,일정표체수평적CHS、CHI야시국화화색소합성소필수적,F3H기인여화색소합성불존재직접상관.
In order to clarify the roles of anthocyanin biosynthetic genes in the anthocyanin biosynthesis and pigmentation of chrysanthemum flower,CHS,CHI,DFR,F3H,F3'H and 3GT in the ray florets from florescences at different development stages were quantified in the red,yellow,pale purple and white-flowered chrysanthemum cultivars.The results showed that expression profiles of the genes are cultivar-dependent and varied at different development levels,and peaked at stage Ⅱ (loosen bud) or Ⅲ (half-opened flower),of which the transcript levels of DFR and 3GT were extremely higher than those in the remaining cultivars.In yellow-flowered chrysanthemum stage Ⅰ and Ⅱ were higher than those at stage Ⅲ and Ⅳ.The transcripts level of 3GT and DFR was higher or lower than that in pale purple cultivar,respectively,but both were lower than that in red among four cultivars studied,while accumulation of F3'H transcripts was near or slightly lower to that of red and pale purple cultivars,and remained stable lesser compared with those of red and yellow cultivars.In white than those in other three colored cultivars.It inferred that DFR and 3GT genes play key roles in the anthocyanin biosynthesis,and DFR is likely the bottle-neck gene in anthocyanin biosynthesis.A certain levels of CHS and CHI transcripts are necessary,the relationship of F3H expression and anthocyanin biosynthesis remained unclear.