CAJ | 학술논문

通过基因芯片技术,利用Roche-NimbleGen公司制作的大鼠12×135K全基因组表达谱芯片,对日龄为6d和10d的大鼠睾丸组织进行全基因组表达差异分析。结果显示：具有2倍以上的差异表达基因有4298个,其中表达上调的基因共1878个,表达下调的基因共2420个。这些差异表达的基因中有3154个基因具有基因本体注释,参与了154个生物学通路。进一步分析表明具有8倍以上差异表达的基因有13个,这些基因参与了生物学过程、细胞组分和分子功能等基因本体分类,进一步选择3个差异表达的基因,LOC686076、Cxcl6和Trib3,做了实时定量RT-PCR检测。其结果趋势与芯片数据一致。因此,我们初步认为精原干细胞的发生与增殖在大鼠早期的发育过程中已经有大量的基因参与,是一个多基因协调表达的过程。
통과기인심편기술,이용Roche-NimbleGen공사제작적대서12×135K전기인조표체보심편,대일령위6d화10d적대서고환조직진행전기인조표체차이분석。결과현시：구유2배이상적차이표체기인유4298개,기중표체상조적기인공1878개,표체하조적기인공2420개。저사차이표체적기인중유3154개기인구유기인본체주석,삼여료154개생물학통로。진일보분석표명구유8배이상차이표체적기인유13개,저사기인삼여료생물학과정、세포조분화분자공능등기인본체분류,진일보선택3개차이표체적기인,LOC686076、Cxcl6화Trib3,주료실시정량RT-PCR검측。기결과추세여심편수거일치。인차,아문초보인위정원간세포적발생여증식재대서조기적발육과정중이경유대량적기인삼여,시일개다기인협조표체적과정。
In this research,differential analysis of genome-wide expression of the rat testis tissues in the age of 6 and 10 days was conducted by gene chip technology based on the 12×135K genome-wide EST microarray of rat produced by Roche-NimbleGen.The results showed that there were 4 298 genes with 2-fold or more differentially expressed in the rat testis tissues between the ages of 6 and 10 days,thereinto,1 878 genes were up-regulated expression and 2 420 genes were down-regulated expression.3 154 genes of these differentially expressed genes have been annotated with gene ontology,participating in the 154 biological pathways.Further analysis showed that there were 13 genes with more than 8 fold differential expression,those genes involved in gene ontology categories,such as biological processes,cellular components and molecular functions and so on.Three differentially expressed genes,LOC686076,Cxcl6 and Trib3,were chosen to detect by real-time quantitative RT-PCR,the results exhibited a tread toward the microarray data.Therefore,we preliminary thought that a large number of genes should be involved in the occurrence and proliferation of spermatogonial stem cell in early stages of growth and development of the rat,it might be a synchronizing process of multi-gene expression.