中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
6期
1154-1156
,共3页
粒细胞集落刺激因子%心肌梗死%动作电位
粒細胞集落刺激因子%心肌梗死%動作電位
립세포집락자격인자%심기경사%동작전위
Granuloeyte colony-stimulating factor%Myocardial infarction%Action potential
目的 观察粒细胞集落刺激因子(G-CSF)对心肌梗死后动作电位的影响.方法 38只大白兔开胸结扎左冠脉前降支,治疗组皮下注射G-CSF每天10μ/kg连续5d,对照组皮下注射每天0.5 ml/kg生理盐水连续5d;观察心肌细胞不同部位不同时间心肌动作电位的变化.结果 在术后30 min、7d、14d、30d、60d两组心肌动作电位在中央区、移行区及边缘区均有下降,但治疗组在移行区较快恢复[治疗组7d (120.0±3.9) ms、14d (136.0±3.3) ms、30d (140.0±4.7) ms；对照组7d (107.0±4.4) ms、14d (112.0±4.6) ms、30d (120.0±7.1) ms,P＜0.05]；左室射血分数(LVEF)治疗组(70.3±9.4)％较对照组(58.2±13.7)％明显升高(P＜0.05),短轴缩短率(FS)治疗组(39.2±6.4)％较对照组(28.711.1)％明显升高(P＜0.05).结论 G-CSF能够加速心肌梗死后心肌动作电位的恢复；从而有效地加速疾病恢复的进程.
目的 觀察粒細胞集落刺激因子(G-CSF)對心肌梗死後動作電位的影響.方法 38隻大白兔開胸結扎左冠脈前降支,治療組皮下註射G-CSF每天10μ/kg連續5d,對照組皮下註射每天0.5 ml/kg生理鹽水連續5d;觀察心肌細胞不同部位不同時間心肌動作電位的變化.結果 在術後30 min、7d、14d、30d、60d兩組心肌動作電位在中央區、移行區及邊緣區均有下降,但治療組在移行區較快恢複[治療組7d (120.0±3.9) ms、14d (136.0±3.3) ms、30d (140.0±4.7) ms；對照組7d (107.0±4.4) ms、14d (112.0±4.6) ms、30d (120.0±7.1) ms,P＜0.05]；左室射血分數(LVEF)治療組(70.3±9.4)％較對照組(58.2±13.7)％明顯升高(P＜0.05),短軸縮短率(FS)治療組(39.2±6.4)％較對照組(28.711.1)％明顯升高(P＜0.05).結論 G-CSF能夠加速心肌梗死後心肌動作電位的恢複；從而有效地加速疾病恢複的進程.
목적 관찰립세포집락자격인자(G-CSF)대심기경사후동작전위적영향.방법 38지대백토개흉결찰좌관맥전강지,치료조피하주사G-CSF매천10μ/kg련속5d,대조조피하주사매천0.5 ml/kg생리염수련속5d;관찰심기세포불동부위불동시간심기동작전위적변화.결과 재술후30 min、7d、14d、30d、60d량조심기동작전위재중앙구、이행구급변연구균유하강,단치료조재이행구교쾌회복[치료조7d (120.0±3.9) ms、14d (136.0±3.3) ms、30d (140.0±4.7) ms；대조조7d (107.0±4.4) ms、14d (112.0±4.6) ms、30d (120.0±7.1) ms,P＜0.05]；좌실사혈분수(LVEF)치료조(70.3±9.4)％교대조조(58.2±13.7)％명현승고(P＜0.05),단축축단솔(FS)치료조(39.2±6.4)％교대조조(28.711.1)％명현승고(P＜0.05).결론 G-CSF능구가속심기경사후심기동작전위적회복；종이유효지가속질병회복적진정.
Objective To observe the effects of granulocyte colony-stimulating factors (G-CSF)on action potential of cardiac myocytes in a rabbit model of acute myocardial infarction.Methods Under anesthesia and mechanical ventilation with room air,a left thoracotomy was performed,and 4-0 silk string was used to ligate the left branch of coronary artery.Thirty-eight healthy New Zealand white rabbits were divided into two groups:G-CSF group and normal saline group.10 μg/(kg·day) G-CSF in G-CSF group and 0.5 ml/(kg·day) normal saline in control group were given 24 h after infarction,one time per day for 5 days.The changes in action potential of cardiac myocytes at different areas and time points after acute myocardial infarction were observed.Results Action potential duration of cardiac myocytes was obviously decreased 30 min,7 days,14 days,30 days,and 60 days after myocardial infarction in different areas in both two groups,but recovered fastly in treatment group [7th day:( 120.0 ±3.9) ms,14th day:( 136.0 ±3.3) ms,30th day:(140.0 ±4.7) ms]as compared with control group in marginal area [7th day:(107.0 ±4.4) ms,14th day:(112.0 ±4.6) ms,30th day:(120.0 ±7.1) ms],P＜0.05.Ejection fraction and fractional shortening were significantly increased in treatment group as compared with control group ( both P ＜ 0.05 ).Conclusion The change of action potential suggests G-CSF may play an important role in the treatment of myocardial infarction.
