中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
5期
562-566
,共5页
赵冠飞%齐福华%王清涛%李永哲
趙冠飛%齊福華%王清濤%李永哲
조관비%제복화%왕청도%리영철
葡萄糖-6-磷酸异构酶%类风湿关节炎%逆转录-聚合酶链反应%酶联免疫吸附试验
葡萄糖-6-燐痠異構酶%類風濕關節炎%逆轉錄-聚閤酶鏈反應%酶聯免疫吸附試驗
포도당-6-린산이구매%류풍습관절염%역전록-취합매련반응%매련면역흡부시험
Glucose-6-phosphate isomerase%Arthritis,rheumatoid%Reverse transcription polymerase chain reaction%Enzyme-linked imnunosorbent assay
目的 探讨类风湿关节炎(BA)患者外周血单个核细胞(PBMC)中葡萄糖-6-磷酸异构酶(GPI)mRNA及血清中GPI抗原表达与RA发病机制及活动性的关系,分析比较GPI与抗突变型瓜氨酸波形蛋白(MCV)抗体、抗环瓜氨酸多肽(CCP)抗体、类风湿因子(RE)在RA诊断中的价值.方法 用实时荧光定量逆转录(FQ-RT)-PCR检测60例RA患者(活动期28例、缓解期32例)、30例其他风湿性疾病患者、30名健康体检者PBMC中GPI mRNA的表达水平和含量[以△Ct=Ct1(待测基因)-Ct2(内参基因)来比较基因表达水平的高低];同时用ELISA检测各组血清中GPI和抗MCV抗体,抗CCP抗体和RF水平.结果 RA患者GPI mRNA表达水平[△Ct=4.21(3.04～7.23)]明显高于其他风湿性疾病组[△Ct=8.42(5.16～9.98),P<0.01]和健康对照组[△Ct=8.66(4.90～10.01),P<0.01],活动期组GPI mRNA表达水平[△Ct=3.78(1.28～6.09)]与缓解期组[△Ct=5.88(3.23～8.94)]的差异有统计学意义(H=11.760,P<0.01);RA组血清GPI水平[3.02(2.02～8.39)mg/L]明显高于其他风湿性疾病组[0.20(0.11～0.32)mg/L]和健康对照组[0.18(0.08～0.30)mg/L],且RA活动期组血清GPI水平[4.84(2.81～10.38)mg/L]与缓解期组[2.12(1.26～4.34)mg/L]差异也有统计学意义(H=9.830,P<0.01).同时检测GPI、抗MCV抗体和抗CCP抗体对RA的敏感度分别为68%(41/60)、57%(34/60)、58%(35/60);特异度分别为95%(57/60)、92%(55/60)、93%(56/60).结论 GPI mRNA可能参与了RA的发病过程,并与疾病的活动性相关,血清中GPI对RA诊断有相对较高的敏感度和特异度,可作为临床诊断RA的辅助指标.
目的 探討類風濕關節炎(BA)患者外週血單箇覈細胞(PBMC)中葡萄糖-6-燐痠異構酶(GPI)mRNA及血清中GPI抗原錶達與RA髮病機製及活動性的關繫,分析比較GPI與抗突變型瓜氨痠波形蛋白(MCV)抗體、抗環瓜氨痠多肽(CCP)抗體、類風濕因子(RE)在RA診斷中的價值.方法 用實時熒光定量逆轉錄(FQ-RT)-PCR檢測60例RA患者(活動期28例、緩解期32例)、30例其他風濕性疾病患者、30名健康體檢者PBMC中GPI mRNA的錶達水平和含量[以△Ct=Ct1(待測基因)-Ct2(內參基因)來比較基因錶達水平的高低];同時用ELISA檢測各組血清中GPI和抗MCV抗體,抗CCP抗體和RF水平.結果 RA患者GPI mRNA錶達水平[△Ct=4.21(3.04～7.23)]明顯高于其他風濕性疾病組[△Ct=8.42(5.16～9.98),P<0.01]和健康對照組[△Ct=8.66(4.90～10.01),P<0.01],活動期組GPI mRNA錶達水平[△Ct=3.78(1.28～6.09)]與緩解期組[△Ct=5.88(3.23～8.94)]的差異有統計學意義(H=11.760,P<0.01);RA組血清GPI水平[3.02(2.02～8.39)mg/L]明顯高于其他風濕性疾病組[0.20(0.11～0.32)mg/L]和健康對照組[0.18(0.08～0.30)mg/L],且RA活動期組血清GPI水平[4.84(2.81～10.38)mg/L]與緩解期組[2.12(1.26～4.34)mg/L]差異也有統計學意義(H=9.830,P<0.01).同時檢測GPI、抗MCV抗體和抗CCP抗體對RA的敏感度分彆為68%(41/60)、57%(34/60)、58%(35/60);特異度分彆為95%(57/60)、92%(55/60)、93%(56/60).結論 GPI mRNA可能參與瞭RA的髮病過程,併與疾病的活動性相關,血清中GPI對RA診斷有相對較高的敏感度和特異度,可作為臨床診斷RA的輔助指標.
목적 탐토류풍습관절염(BA)환자외주혈단개핵세포(PBMC)중포도당-6-린산이구매(GPI)mRNA급혈청중GPI항원표체여RA발병궤제급활동성적관계,분석비교GPI여항돌변형과안산파형단백(MCV)항체、항배과안산다태(CCP)항체、류풍습인자(RE)재RA진단중적개치.방법 용실시형광정량역전록(FQ-RT)-PCR검측60례RA환자(활동기28례、완해기32례)、30례기타풍습성질병환자、30명건강체검자PBMC중GPI mRNA적표체수평화함량[이△Ct=Ct1(대측기인)-Ct2(내삼기인)래비교기인표체수평적고저];동시용ELISA검측각조혈청중GPI화항MCV항체,항CCP항체화RF수평.결과 RA환자GPI mRNA표체수평[△Ct=4.21(3.04～7.23)]명현고우기타풍습성질병조[△Ct=8.42(5.16～9.98),P<0.01]화건강대조조[△Ct=8.66(4.90～10.01),P<0.01],활동기조GPI mRNA표체수평[△Ct=3.78(1.28～6.09)]여완해기조[△Ct=5.88(3.23～8.94)]적차이유통계학의의(H=11.760,P<0.01);RA조혈청GPI수평[3.02(2.02～8.39)mg/L]명현고우기타풍습성질병조[0.20(0.11～0.32)mg/L]화건강대조조[0.18(0.08～0.30)mg/L],차RA활동기조혈청GPI수평[4.84(2.81～10.38)mg/L]여완해기조[2.12(1.26～4.34)mg/L]차이야유통계학의의(H=9.830,P<0.01).동시검측GPI、항MCV항체화항CCP항체대RA적민감도분별위68%(41/60)、57%(34/60)、58%(35/60);특이도분별위95%(57/60)、92%(55/60)、93%(56/60).결론 GPI mRNA가능삼여료RA적발병과정,병여질병적활동성상관,혈청중GPI대RA진단유상대교고적민감도화특이도,가작위림상진단RA적보조지표.
Objective To assess the levels of glucose-6-phosphate isomerase(GPI) mRNA in peripheral blood monocytes and serum GPI levels in patients with rheumatoid arthritis, and analyze the association of serum GPI with MCV antibody, CCP antibody and RF of RA. Methods Fluorogenic quantitative real-time polymerase chain reaction (FQ-RT-PCR) was used to examine mRNA expression on peripheral blood monocytes in 60 RA patients (28 case in active stage,32 cases in stable stage) ,30 patients with other rheumatic diseases, and 30 healthy controls. ELISA was used to detect the levels of serum GPI, anti-MCV antibodies, anti-CCP antibodies and RF in each group. Results The levels of GPI mRNA in RA group [△Ct=4.21 (3.04-7.23)] were significantly higher than those in patients with other rheumatic diseases [△Ct=8.42 (5.16-9.98),P<0.01] and healthy controls [△Ct=8.66 (4.90-10.01), P<0.01]. There were statistically significant differences of GPI mRNA levels between active RA [△Ct=3.78 (1.28-6.09)] and inactive RA[△Ct =5.88(3.23-8.94),H=11.760,P<0.01)]. The RA group serum GPI levels [3.02 (2.02-8.39) mg/L] were higher than those of other rheumatic diseases [0.20 (0.11-0.32) mg/L] and healthy controls [0.18(0.08-0.30) mg/L]. There were significant differences of serum GPI levels between active RA group [4.84(2.81-10.38) mg/L] and inactive RA group[2.12 (1.26-4.34) mg/L] (H=9.830, P<0.01). The sensitivities of GPI, anti-MCV and anti-CCP were 68% (41/60) ,57% (34/60),58% (35/60), respectively and specificities were 95% (57/60), 92% (55/60) and 93% (56/60), respectively. Conclusions The high expression of GPI mRNA in RA patients shows that it may play a pathological role in the development of RA, and it may be correlated with the activity of RA. It may be a valuable diagnostic parameter for RA, because of its high sensitivity and specificity.
