CAJ | 학술논문

目的应用微小免疫磁珠分离提纯SD胎鼠大脑皮层组织中的神经干细胞并进行培养,为研究神经干细胞的特性与神经干细胞的培养和移植研究创造有利条件.方法取SD胎鼠的大脑皮层组织制成单细胞悬液,用微小磁珠(平均直径≤50 am)分选出神经巢蛋白(nestin)阳性的细胞群,以流式细胞术检测阳性细胞纯度,以台盼蓝染色法检测细胞活性.结果分离的神经干细胞流式细胞仪检测细胞nestin阳性表达率为(88.5±3.6)%,细胞存活率为(96.3±1.8)%.结论微小免疫磁珠法分离胎鼠脑神经干细胞群落简便、有效,可以为神经干细胞的细胞培养和移植提供细胞来源.
목적 응용미소면역자주분리제순SD태서대뇌피층조직중적신경간세포병진행배양,위연구신경간세포적특성여신경간세포적배양화이식연구창조유리조건.방법 취SD태서적대뇌피층조직제성단세포현액,용미소자주(평균직경≤50 am)분선출신경소단백(nestin)양성적세포군,이류식세포술검측양성세포순도,이태반람염색법검측세포활성.결과 분리적신경간세포류식세포의검측세포nestin양성표체솔위(88.5±3.6)%,세포존활솔위(96.3±1.8)%.결론 미소면역자주법분리태서뇌신경간세포군락간편、유효,가이위신경간세포적세포배양화이식제공세포래원.
Objective To isolate the neural stem cells from the fetal rat brain by immunomagnetic beads technique(d≤50 nm),and then to culture purified cells for studying the property of these cells.Methods Cell suspension of the fetal rat cerebral cortex was incubated with antibody of nestin and these labeled cells were separated from the suspension through the magnetic field by immunomagnetic beads coated with the second antibody.Purity of the sorted cells was determined with flow cytometry.Results Purity of the sorted neural stem cells were determined as(88.5±3.6)%,viable cells account for(96.3±1.8 1%.Conclusion The magnetic cell sorting system can separate neural stem cells effectively from brain cell suspension.