CAJ | 학술논문

利用发根农杆菌A4菌株在西洋参根外植体上直接诱导产生发根.在1/2 MS和MS固体培养基上得到西洋参发根,并在B5液体培养基上建立起发根离体培养系,经连续多代的培养,发根仍保持旺盛生长状态.PCR扩增结果表明,发根农杆菌Ri质粒的rolC基因已整合到西洋参发根基因组中并得到表达.利用紫外分光光度法测得西洋参发根的总皂苷含量达3.88%.经对发根比生长速率的测定,确定1/2 B5培养基(15 g/L蔗糖)、摇床转速100 r/min、每4周更换1次培养基并继代培养1次为西洋参发根生长适宜条件.
이용발근농간균A4균주재서양삼근외식체상직접유도산생발근.재1/2 MS화MS고체배양기상득도서양삼발근,병재B5액체배양기상건립기발근리체배양계,경련속다대적배양,발근잉보지왕성생장상태.PCR확증결과표명,발근농간균Ri질립적rolC기인이정합도서양삼발근기인조중병득도표체.이용자외분광광도법측득서양삼발근적총조감함량체3.88%.경대발근비생장속솔적측정,학정1/2 B5배양기(15 g/L자당)、요상전속100 r/min、매4주경환1차배양기병계대배양1차위서양삼발근생장괄의조건.
Hairy roots of Panax quinquefolium L. were induced from the root explants of two-year old Panax quinquefolium L. by Agrobacterium rhizogenes A4 with direct inoculation, the cultured clones of the hairy roots were established on solid 1/2 MS and MS mediums. After several times of subcultures, the hairy roots still maintained vigorous growth. After the determination of UV-spectrophotometry, the total ginsenosides content in the hairy root came up to 3.88%. The suitable culture conditions for Panax quinquefolium L. hairy roots growing were 1/2 B5 liquid medium(15 g/L glucose), in a shaker at 100 r/min, changing the culture solution and subculturing the hairy roots every 4 weeks.