CAJ | 학술논문

建立了HPLC法同时测定六味地黄丸中的丹皮酚、马钱苷、莫诺苷和芍药苷.采用C_(18)色谱柱,以乙腈-0.1%磷酸溶液梯度洗脱,检测波长238 nm,丹皮酚、马钱苷、莫诺苷和芍药苷分别在0.5～20、0.25～10、0.5～20和0.15～6μg/ml浓度范围内线性关系良好,回收率分别为98.10%～105.44%.
건립료HPLC법동시측정륙미지황환중적단피분、마전감、막낙감화작약감.채용C_(18)색보주,이을정-0.1%린산용액제도세탈,검측파장238 nm,단피분、마전감、막낙감화작약감분별재0.5～20、0.25～10、0.5～20화0.15～6μg/ml농도범위내선성관계량호,회수솔분별위98.10%～105.44%.
An HPLC method was established for the simultaneous determination of paeonol,loganin, morroniside and paeoniflorin in Liuwei Dihuang pills.A C_(18) column was used with the mobile phase of acetonitrile-0.1% phosphoric acid for gradient elution at the detection wavelength of 238 nm.The calibration curves of the four constituents were linear in the concentration ranges of 0.5-20,0.25-10,0.5-20 and 0.15-6 μg/ml,respectively.The recoveries were 98.10%-105.44%.