CAJ | 학술논문

目的研制对氧磷酶长循环脂质体.方法采用薄膜分散法制备对氧磷酶长循环脂质体,采用凝胶柱法测定包封率,以包封率为指标,分别考察药脂比、胆固醇用量、聚乙二醇-胆固醇用量、离子强度等对脂质体的影响,在此基础上用正交设计对处方进行优化.结果经薄膜分散法制得的脂质体包封率为(87.66±3.46)%,平均粒径为126 nm左右,粒度分布均匀,呈单峰分布,电镜结果显示外形圆整,分散性较好.4℃放置15 d包封率无明显变化,酶活性基本稳定.结论聚乙二醇修饰的对氧磷酶长循环脂质体制备工艺简单可行,对氧磷酶长循环脂质体制剂学、酶学性质稳定.
목적 연제대양린매장순배지질체.방법 채용박막분산법제비대양린매장순배지질체,채용응효주법측정포봉솔,이포봉솔위지표,분별고찰약지비、담고순용량、취을이순-담고순용량、리자강도등대지질체적영향,재차기출상용정교설계대처방진행우화.결과 경박막분산법제득적지질체포봉솔위(87.66±3.46)%,평균립경위126 nm좌우,립도분포균균,정단봉분포,전경결과현시외형원정,분산성교호.4℃방치15 d포봉솔무명현변화,매활성기본은정.결론 취을이순수식적대양린매장순배지질체제비공예간단가행,대양린매장순배지질체제제학、매학성질은정.
Objective To prepare the long-circulating liposomes of paraoxonase(PON).Methods The long-circulating liposomes of paraoxonase were prepared by film dispersion method.The encapsulation efficiency was determined by gel column.The effects of the factors on the encapsulation efficiency,such as the weight ratio of paraoxonase to phospholipid,cholesterol(Choi) to phospholipid,PEG-cholesterol (PEG-Chol) and the iron strength of water phase,were investigated respectively.Then the formulation was optimized by orthogonal design.Results The encapsulation efficiency of the paraoxonase liposomes was 87.66±3.46%,and the average diameter of the liposomes was about 126 nm.There was no significant change on encapsulation efficiency on 15 d at 4 ℃,and the activity of paraoxonase was maintained basically stable.Conclusion The preparation of PEG-modified paraoxonase liposomes was easy and practicable,and the property investigation in vitro showed that the paraoxonase liposomes were stable.