辐射损伤,实验性%坐骨神经%放射学,介入性%放射性同位素%动物,实验
輻射損傷,實驗性%坐骨神經%放射學,介入性%放射性同位素%動物,實驗
복사손상,실험성%좌골신경%방사학,개입성%방사성동위소%동물,실험
Radiation injuries,experimental%Sciatic nerve%Radiology,interventional%Radioisotopes%Animals,laboratory
目的 观察125I粒子植入后不同时间家兔坐骨神经组织形态及功能的变化.方法 选健康新西兰家兔30只,使用信封法分为2周、2个月及4个月3组,每组10只,直视下在兔的实验侧坐骨神经旁植入125I粒子10粒,对照侧植入无放射活性空粒子10粒.按三维治疗计划系统(treatment plan system,TPS)计划设计布源,90%处方剂量集中在所研究的坐骨神经局部.术后2周、2个月及4个月行双侧坐骨神经神经电生理测定及大体观察、光镜观察和电镜观察.将同为4000放大倍数的电镜照片分为100(10×10)个方格,出现非特异性改变占1个方格则计数为1%.所测指标之间的比较采用t检验和秩和检验.结果 2周、 2个月、4个月组兔实验侧坐骨神经的近心端动作电位强度分别为(0.52±0.26)、(0.60±0.19)、(0.48±0.17)V,对照侧分别为(0.59±0.19)、(0.60±0.15)、(0.53±0.13)V,差异无统计学意义(t值分别为0.91、0.03、0.67,P值均>0.05),2周、2个月、4个月组兔实验侧坐骨神经远心端动作电位强度分别为(0.51±0.15)、(0.52±0.11)、(0.53±0.15)V,对照侧分别为(0.52±0.10)、(0.56±0.12)、(0.54±0.10)V,差异无统计学意义(t值分别为0.25、0.74、0.17,P值均>0.05);2周、2个月、4个月组兔实验侧坐骨神经近心端动作电位最大振幅分别为(13.18±4.09)、(12.78±4.42)、(12.09±1.20)mV,对照侧分别为(10.55±4.21)、(10.31±4.22)、(12.88±3.54)mV,差异无统计学意义(t值分别为1.57、1.36、0.50,P值均>0.05),2周、2个月、4个月组兔实验侧坐骨神经的远心端动作电位最大振幅分别为(11.18±3.38)、(11.68±3.21)、(12.52±3.09)mV,对照侧分别为(11.56±4.80)、(10.71±3.40)、(11.67±2.48)mV,差异无统计学意义(t值分别为0.29、1.01、0.55,P值均>0.05);2周、2个月、4个月组兔实验侧坐骨神经神经传导速度分别为(40.56±9.46)、(38.79±5.78)、(39.44±8.64)m/V,对照侧为(42.56±6.59)、(44.64±7.53)、(43.33±6.05)m/V,差异无统计学意义(t值分别为0.57、1.94、0.01,P值均>0.05).大体学观察和光镜观察实验侧坐骨神经病理学改变不明显;电镜观察可见到有髓神经鞘分层、塌陷、崩解等变性改变;神经鞘膜细胞和神经轴突内可见线粒体肿胀、空泡化.非特异性变化2周组为60%-70%,2个月组为50%左右,而4个月组下降到30%左右,3组比较差异有统计学意义(Z值均为-3.79,P<0.05).结论 该实验剂量下放射性125I粒子对家兔坐骨神经的组织影响以超微病理下的非特异性变化为主,对神经的生理功能影响微小.
目的 觀察125I粒子植入後不同時間傢兔坐骨神經組織形態及功能的變化.方法 選健康新西蘭傢兔30隻,使用信封法分為2週、2箇月及4箇月3組,每組10隻,直視下在兔的實驗側坐骨神經徬植入125I粒子10粒,對照側植入無放射活性空粒子10粒.按三維治療計劃繫統(treatment plan system,TPS)計劃設計佈源,90%處方劑量集中在所研究的坐骨神經跼部.術後2週、2箇月及4箇月行雙側坐骨神經神經電生理測定及大體觀察、光鏡觀察和電鏡觀察.將同為4000放大倍數的電鏡照片分為100(10×10)箇方格,齣現非特異性改變佔1箇方格則計數為1%.所測指標之間的比較採用t檢驗和秩和檢驗.結果 2週、 2箇月、4箇月組兔實驗側坐骨神經的近心耑動作電位彊度分彆為(0.52±0.26)、(0.60±0.19)、(0.48±0.17)V,對照側分彆為(0.59±0.19)、(0.60±0.15)、(0.53±0.13)V,差異無統計學意義(t值分彆為0.91、0.03、0.67,P值均>0.05),2週、2箇月、4箇月組兔實驗側坐骨神經遠心耑動作電位彊度分彆為(0.51±0.15)、(0.52±0.11)、(0.53±0.15)V,對照側分彆為(0.52±0.10)、(0.56±0.12)、(0.54±0.10)V,差異無統計學意義(t值分彆為0.25、0.74、0.17,P值均>0.05);2週、2箇月、4箇月組兔實驗側坐骨神經近心耑動作電位最大振幅分彆為(13.18±4.09)、(12.78±4.42)、(12.09±1.20)mV,對照側分彆為(10.55±4.21)、(10.31±4.22)、(12.88±3.54)mV,差異無統計學意義(t值分彆為1.57、1.36、0.50,P值均>0.05),2週、2箇月、4箇月組兔實驗側坐骨神經的遠心耑動作電位最大振幅分彆為(11.18±3.38)、(11.68±3.21)、(12.52±3.09)mV,對照側分彆為(11.56±4.80)、(10.71±3.40)、(11.67±2.48)mV,差異無統計學意義(t值分彆為0.29、1.01、0.55,P值均>0.05);2週、2箇月、4箇月組兔實驗側坐骨神經神經傳導速度分彆為(40.56±9.46)、(38.79±5.78)、(39.44±8.64)m/V,對照側為(42.56±6.59)、(44.64±7.53)、(43.33±6.05)m/V,差異無統計學意義(t值分彆為0.57、1.94、0.01,P值均>0.05).大體學觀察和光鏡觀察實驗側坐骨神經病理學改變不明顯;電鏡觀察可見到有髓神經鞘分層、塌陷、崩解等變性改變;神經鞘膜細胞和神經軸突內可見線粒體腫脹、空泡化.非特異性變化2週組為60%-70%,2箇月組為50%左右,而4箇月組下降到30%左右,3組比較差異有統計學意義(Z值均為-3.79,P<0.05).結論 該實驗劑量下放射性125I粒子對傢兔坐骨神經的組織影響以超微病理下的非特異性變化為主,對神經的生理功能影響微小.
목적 관찰125I입자식입후불동시간가토좌골신경조직형태급공능적변화.방법 선건강신서란가토30지,사용신봉법분위2주、2개월급4개월3조,매조10지,직시하재토적실험측좌골신경방식입125I입자10립,대조측식입무방사활성공입자10립.안삼유치료계화계통(treatment plan system,TPS)계화설계포원,90%처방제량집중재소연구적좌골신경국부.술후2주、2개월급4개월행쌍측좌골신경신경전생리측정급대체관찰、광경관찰화전경관찰.장동위4000방대배수적전경조편분위100(10×10)개방격,출현비특이성개변점1개방격칙계수위1%.소측지표지간적비교채용t검험화질화검험.결과 2주、 2개월、4개월조토실험측좌골신경적근심단동작전위강도분별위(0.52±0.26)、(0.60±0.19)、(0.48±0.17)V,대조측분별위(0.59±0.19)、(0.60±0.15)、(0.53±0.13)V,차이무통계학의의(t치분별위0.91、0.03、0.67,P치균>0.05),2주、2개월、4개월조토실험측좌골신경원심단동작전위강도분별위(0.51±0.15)、(0.52±0.11)、(0.53±0.15)V,대조측분별위(0.52±0.10)、(0.56±0.12)、(0.54±0.10)V,차이무통계학의의(t치분별위0.25、0.74、0.17,P치균>0.05);2주、2개월、4개월조토실험측좌골신경근심단동작전위최대진폭분별위(13.18±4.09)、(12.78±4.42)、(12.09±1.20)mV,대조측분별위(10.55±4.21)、(10.31±4.22)、(12.88±3.54)mV,차이무통계학의의(t치분별위1.57、1.36、0.50,P치균>0.05),2주、2개월、4개월조토실험측좌골신경적원심단동작전위최대진폭분별위(11.18±3.38)、(11.68±3.21)、(12.52±3.09)mV,대조측분별위(11.56±4.80)、(10.71±3.40)、(11.67±2.48)mV,차이무통계학의의(t치분별위0.29、1.01、0.55,P치균>0.05);2주、2개월、4개월조토실험측좌골신경신경전도속도분별위(40.56±9.46)、(38.79±5.78)、(39.44±8.64)m/V,대조측위(42.56±6.59)、(44.64±7.53)、(43.33±6.05)m/V,차이무통계학의의(t치분별위0.57、1.94、0.01,P치균>0.05).대체학관찰화광경관찰실험측좌골신경병이학개변불명현;전경관찰가견도유수신경초분층、탑함、붕해등변성개변;신경초막세포화신경축돌내가견선립체종창、공포화.비특이성변화2주조위60%-70%,2개월조위50%좌우,이4개월조하강도30%좌우,3조비교차이유통계학의의(Z치균위-3.79,P<0.05).결론 해실험제량하방사성125I입자대가토좌골신경적조직영향이초미병리하적비특이성변화위주,대신경적생리공능영향미소.
Objective To study the effect of 125Iodine seed on the rabbit ischiadic nerve at different time point after implantation. Methods Thirty healthy New-Zealand rabbits were randomly assigned into 3 groups( 2-week group, 2-month group and 4-month group) using envelope method. During operation, 10 radioactive 125I seeds were implanted randomly near one of the ischiadic nerve, while 10 non-radioactive seeds were implanted into the contralateral ischiadie nerve. According to treatment plan system(TPS),90% of the prescription dose (PD)was centered in the specific place, where the nerves were chosen to be studied. After 2 weeks, 2 months and 4 months respectively, nerve electro-physiolngy experiment was used to evaluate the bilateral ischiadic nerves, at the same time the morphology of the ischiadic nerve was examined by general observation, light microscope and electron microscope. The electron microscope photo with the same ×4000 amplification was divided into 100( 10 × 10) cages and non-specific changes in one cage account for 1%. The t test and sum rank test were used for statistics. Results Potential leaking point of experimental ischiadic nerves near heart in 2-week group ,2-month group and 4-month group were (0.52± 0.26), (0.60±0.19), (0.48±0.17)V, while that of the control sides were (0.59±0.19), (0.60± 0.15), (0.53±0.13 ) V, there was no statistical significance in the same group respectively (t=0.91, 0.03,0.67,P>0.05). Potential leak point of experimental ischiadic nerves far from heart in 2-week group, 2-month group and 4-month group were (0.51±0.15), (0.52 s0. 11 ), (0. 53±0.15) V,the control sides were (0.52±0.10), (0.56±0.12), (0.54±0.10)V, there was no statistical significance in the same group respectively (t= 0.25,0.74,0.17, P > 0.05 ). Action potential amplitude of experimental ischiadic nerves near heart in 2-week group,2-menth group and 4-month group were (13.18±4.09), (12.78± 4.42), (12.09±1.20) mV, while that of the control sides were (10.55± 4.21 ), ( 10.31±4.22), (12.88±3.54) mV, there was no statistical significance in the same group respectively (t=1.57,1.36, 0.50,P>0.05). Action potential amplitude of experimental ischiadic nerves far from heart in 2-week group,2-month group and 4-month group were (11.18±3.38), (11.68±3.21), ( 12.52±3.09) mV, while that of the control sides were (11.56±4.80), (10.71±3.40), (11.67±2.48) mV ,there was no statistical significance in the same group respectively(t=0.29,1.01,0.55, P>0.05 ). Nerve conduction velocity of experimental ischiadic nerves in 2-week group,2-month group and 4-month group were (40.56± 9.46), (38.79±5.78), (39.44±8.64) m/V, the control sides were (42.56±6.59), (44.64±7.53), (43.33±6.05)m/V, there was no statistical significance in the same group respectively( t = 0.57,1.94, 0.01,P>0.05). There were some changes in general observation and light microscope, in electron microscope, many non-specificity changes were observed. All of these changes included delamination, collapse, disaggregation of the myelinated nerve, mitochondria swelling and vacuolization of neurilemma cell and axon. The ratio of degenerative alterations in nerves was 60% --70% in 2-week group, 50% in 2-month group and 30% in 4-month group, and there was statistical significance among three groups (P<0.05). Conclusion 125I permanent plantation in our test dose has little effect on ischiadic nerve, all these non-specificity changes were observed in electron microscope, and it has no evident impacts on physiological functions.
