中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
4期
330-334
,共5页
郑晓慧%高雪%李妍%刘丽文%易小敏%陈磊%赵永峰%宋宏萍%张荣庆%王海昌
鄭曉慧%高雪%李妍%劉麗文%易小敏%陳磊%趙永峰%宋宏萍%張榮慶%王海昌
정효혜%고설%리연%류려문%역소민%진뢰%조영봉%송굉평%장영경%왕해창
低温%应激%腹腔动脉%动脉硬化%新生血管化,病理性
低溫%應激%腹腔動脈%動脈硬化%新生血管化,病理性
저온%응격%복강동맥%동맥경화%신생혈관화,병이성
Hypothermia%Stress%Celiac artery%Arteriosclerosis%Neovascularization,pathologic
目的 探讨建立冷应激家兔腹主动脉粥样硬化不稳定斑块模型的方法和评价. 方法60只雄性纯种新西兰大白兔,数字抽签随机分为3组(每组20只).冷应激组高脂喂养加球囊损伤,此外除手术后1周每天置于4℃环境中给予冷应激刺激1h,持续到第20周；球囊损伤组单纯高脂喂养加球囊损伤；对照组普通颗粒饲料喂养无特殊处理.20周末观察血脂、炎性因子、斑块形态、斑块组成成分及新生血管形成的变化. 结果 冷应激组与球囊损伤组兔血脂比较.差异无统计学意义.冷应激组氧化型低密度脂蛋白(ox-LDL),超敏C反应蛋白(hs-CRP)、白细胞介素(IL)-8分别为(56.1±14.3)mg/L、(149.1±78.3)mg/L、(97.6±17.9)μg/L,高于球囊损伤组(42.9±13.8)mg/L、(94.5±57.3)mg/L、(57.5±18.3)μg/L,差异有统计学意义(t值分别为2.78、6.91、14.94,均P＜0.05)；冷应激组巨噬细胞含量为(30.9±5.6)％,高于球囊损伤组(18.7±4.8)％,差异有统计学意义(t=6.88,P＜0.05)；斑块内新生血管生成率冷应激组74.1％(23/31),高于球囊损伤组的20.0％(5/20),差异有统计学意义(x2=16.26,P＜0.05),对照组无斑块形成. 结论 冷应激结合高脂饮食加球囊损伤可成功建立兔动脉硬化不稳定斑块模型,其方法优于传统单纯高脂饮食加球囊损伤.
目的 探討建立冷應激傢兔腹主動脈粥樣硬化不穩定斑塊模型的方法和評價. 方法60隻雄性純種新西蘭大白兔,數字抽籤隨機分為3組(每組20隻).冷應激組高脂餵養加毬囊損傷,此外除手術後1週每天置于4℃環境中給予冷應激刺激1h,持續到第20週；毬囊損傷組單純高脂餵養加毬囊損傷；對照組普通顆粒飼料餵養無特殊處理.20週末觀察血脂、炎性因子、斑塊形態、斑塊組成成分及新生血管形成的變化. 結果 冷應激組與毬囊損傷組兔血脂比較.差異無統計學意義.冷應激組氧化型低密度脂蛋白(ox-LDL),超敏C反應蛋白(hs-CRP)、白細胞介素(IL)-8分彆為(56.1±14.3)mg/L、(149.1±78.3)mg/L、(97.6±17.9)μg/L,高于毬囊損傷組(42.9±13.8)mg/L、(94.5±57.3)mg/L、(57.5±18.3)μg/L,差異有統計學意義(t值分彆為2.78、6.91、14.94,均P＜0.05)；冷應激組巨噬細胞含量為(30.9±5.6)％,高于毬囊損傷組(18.7±4.8)％,差異有統計學意義(t=6.88,P＜0.05)；斑塊內新生血管生成率冷應激組74.1％(23/31),高于毬囊損傷組的20.0％(5/20),差異有統計學意義(x2=16.26,P＜0.05),對照組無斑塊形成. 結論 冷應激結閤高脂飲食加毬囊損傷可成功建立兔動脈硬化不穩定斑塊模型,其方法優于傳統單純高脂飲食加毬囊損傷.
목적 탐토건립랭응격가토복주동맥죽양경화불은정반괴모형적방법화평개. 방법60지웅성순충신서란대백토,수자추첨수궤분위3조(매조20지).랭응격조고지위양가구낭손상,차외제수술후1주매천치우4℃배경중급여랭응격자격1h,지속도제20주；구낭손상조단순고지위양가구낭손상；대조조보통과립사료위양무특수처리.20주말관찰혈지、염성인자、반괴형태、반괴조성성분급신생혈관형성적변화. 결과 랭응격조여구낭손상조토혈지비교.차이무통계학의의.랭응격조양화형저밀도지단백(ox-LDL),초민C반응단백(hs-CRP)、백세포개소(IL)-8분별위(56.1±14.3)mg/L、(149.1±78.3)mg/L、(97.6±17.9)μg/L,고우구낭손상조(42.9±13.8)mg/L、(94.5±57.3)mg/L、(57.5±18.3)μg/L,차이유통계학의의(t치분별위2.78、6.91、14.94,균P＜0.05)；랭응격조거서세포함량위(30.9±5.6)％,고우구낭손상조(18.7±4.8)％,차이유통계학의의(t=6.88,P＜0.05)；반괴내신생혈관생성솔랭응격조74.1％(23/31),고우구낭손상조적20.0％(5/20),차이유통계학의의(x2=16.26,P＜0.05),대조조무반괴형성. 결론 랭응격결합고지음식가구낭손상가성공건립토동맥경화불은정반괴모형,기방법우우전통단순고지음식가구낭손상.
Objective To establish and evaluate unstable atherosclerotic plaque model in abdominal aorta induced by cold stress. Methods Sixty male New Zealand white rabbits were randomly divided into three groups:cold stress group fed with high fat diet and followed by balloon induced arterial wall injury of abdominal aorta at week 2 and exposed to cold (4℃) for 1 h per day except for the first postoperative week,balloon-injury group treated by high fat diet plus balloon-injury, control group fed a normal chow without any treatment. Pathological changes of atherosclerotic plaques among these groups were evaluated at 20 weeks. Meanwhile, serum concentrations of blood lipid,oxidized low density protein(ox-LDL),hypersensitive C-reaction protein (hs-CRP)and interleukin (IL)-8 were determined. Results There was no difference in blood lipid level between cold-stress and balloon-injury groups.Serum concentrations of ox-LDL[(56.1 +14.3)mg/L vs.(42.9± 13.8)mg/L],hs-CRP[(149.1+78.3)mg/L vs. (94.5±57.3)mg/L],IL-8 [(97.6±17.9)μg/L vs.(57.5±18.3)μg/L]and macrophage infiltration[(30.9±5.6)％ vs,(18.7±4.8) ％] were significantly higher in cold stress group than in balloon-injury group (t =2.78,6.91,14.94,6.88,all P＜0.05). Higher angiogenesis rate of atherosclerotic plaque in cold-stress group (23/31,74.1％) was observed in comparison with group balloon-injury(5/25,20,0％)(x2=16.26,P＜0.05). Conclusions Establishment of rabbit unstable atherosclerotic plaque model induced by cold stress in synergy with high fat diet and balloon-injury is feasible, which is superior to conventional method through high fat diet plus balloon-injury surgery.
